Volume 44, Issue 4 (July 1999)

    Simultaneous Detection of Multiple STR Loci on Sex Chromosomes for Forensic Testing of Sex and Identity

    (Received 4 June 1998; accepted 19 October 1998)

    CODEN: JFSOAD

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    Abstract

    The forensic usefulness of X and Y chromosomal STR loci has recently been demonstrated. One quadruplex-PCR, using 2 X- and 2 Y-STRs (STRX1/HPRTB and DYS390/DYS393), and 2 duplex-PCRs, each using an X- and a Y-STR (ARA/DYS390 and ARA/DYS393), and detection of PCR products by using an automated DNA sequencer are reported herein. This approach allows us to determine not only the sex of the donor of a sample, but also the X- and/or Y-STR genotypes of the sample. A male biological specimen yields 4 amplified products in quadruplex-PCR and 2 amplified fragments in duplex-PCRs, whereas a female biological specimen yields only 2 amplified fragments of X-STR in quadruplex-PCR and one fragment, also of X-STR, in duplex-PCRs. Our study thus provides useful information for many activities in forensic practice, such as identity testing, paternity testing, especially of deficiency cases, compilation of population data, and sex determination of a biological sample from a single PCR.


    Author Information:

    Kuroki, H
    Research assistant, Osaka University Graduate School of Medical, Osaka,

    Honda, K
    Associate professor, Osaka University Graduate School of Medical, Osaka,

    Nakamura, M
    Postgraduate student, Osaka University Graduate School of Medical, Osaka,

    Nakatome, M
    Research assistant, Osaka University Graduate School of Medical, Osaka,

    Terada, M
    Forensic toxicologist, Osaka University Graduate School of Medical, Osaka,

    Yamazaki, M
    Research assistant, Osaka University Graduate School of Medical, Osaka,

    Matoba, R
    Professor, Osaka University Graduate School of Medical, Osaka,

    Shimada, S
    Postgraduate student, Osaka University Graduate School of Medical, Osaka,

    Ogura, Y
    Forensic technician, Osaka University Graduate School of Medical, Osaka,

    Tun, Z
    Postgraduate student, Osaka University Graduate School of Medical F3, Suita Osaka,


    Stock #: JFS14552J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14552J

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    Title Simultaneous Detection of Multiple STR Loci on Sex Chromosomes for Forensic Testing of Sex and Identity
    Symposium , 0000-00-00
    Committee E30