ISSN: 0022-1198
CODEN: JFSCA
Page Count: 6

Simultaneous Detection of Multiple STR Loci on Sex Chromosomes for Forensic Testing of Sex and Identity
Kuroki, H
Research assistant, Osaka University Graduate School of Medical,

Honda, K
Associate professor, Osaka University Graduate School of Medical,

Nakamura, M
Postgraduate student, Osaka University Graduate School of Medical,

Nakatome, M
Research assistant, Osaka University Graduate School of Medical,

Terada, M
Forensic toxicologist, Osaka University Graduate School of Medical,

Yamazaki, M
Research assistant, Osaka University Graduate School of Medical,

Matoba, R
Professor, Osaka University Graduate School of Medical,

Shimada, S
Postgraduate student, Osaka University Graduate School of Medical,

Ogura, Y
Forensic technician, Osaka University Graduate School of Medical,

Tun, Z
Postgraduate student, Osaka University Graduate School of Medical F3,

(Received 4 June 1998; accepted 19 October 1998)

Abstract

The forensic usefulness of X and Y chromosomal STR loci has recently been demonstrated. One quadruplex-PCR, using 2 X- and 2 Y-STRs (STRX1/HPRTB and DYS390/DYS393), and 2 duplex-PCRs, each using an X- and a Y-STR (ARA/DYS390 and ARA/DYS393), and detection of PCR products by using an automated DNA sequencer are reported herein. This approach allows us to determine not only the sex of the donor of a sample, but also the X- and/or Y-STR genotypes of the sample. A male biological specimen yields 4 amplified products in quadruplex-PCR and 2 amplified fragments in duplex-PCRs, whereas a female biological specimen yields only 2 amplified fragments of X-STR in quadruplex-PCR and one fragment, also of X-STR, in duplex-PCRs. Our study thus provides useful information for many activities in forensic practice, such as identity testing, paternity testing, especially of deficiency cases, compilation of population data, and sex determination of a biological sample from a single PCR.

Keywords:
forensic science, sex determination, DNA typing, polymerase chain reaction, multiplex, X chromosome, Y chromosome, short tandem repeats

Paper ID: JFS14552J
DOI: 10.1520/JFS14552J
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