Volume 44, Issue 3 (May 1999)

    Removal of a PCR Inhibitor and Resolution of DNA STR Types in Mixed Human-Canine Stains from a Five Year Old Case

    (Received 21 May 1998; accepted 8 September 1998)

    CODEN: JFSOAD

      Format Pages Price  
    PDF Version 4 $25   ADD TO CART


    Abstract

    The analysis of biological trace evidence from a reopened investigation into a 1991 murder from Vernon, B.C. revealed mixed human and dog bloodstains on blue jean pants that contained a PCR inhibitory substance. The presence of the inhibitory substance was detected by the inhibition caused from adding a small aliquot of the test DNA extract into a PCR reaction designed to produce a known standard product. The removal of the PCR inhibitory substance was accomplished by treating the extracted DNA with Thiopropyl Sepharose 6B beads. DNA profiles from two human contributors and a canine were obtained using species specific polymorphic STR markers. The two human DNA profiles obtained from blue jean pants were resolved, one matched the suspect and the other matched the victim. The DNA profile from the canine component matched that obtained from the known sample of the victim's dog who was also slain during the assault. This evidence along with other DNA typing evidence was critical in obtaining a resolution of the case.


    Author Information:

    Johnston, E
    PE AgGen (A Division of PE Applied Biosystems), Davis, California

    Shutler, GG
    I/C Biology Section R.C.M. Police Forensic Laboratory Winnipeg, Winnipeg, Manitoba

    Verret, G

    Kalyn, H
    RCMP Forensic Laboratory Vancouver,

    Halverson, J
    PE AgGen (A Division of PE Applied Biosystems), Davis, California

    Gagnon, P
    RCMP Central Forensic Laboratory, Ottawa,

    Korkosh, S
    I/C Biology Section R.C.M. Police Forensic Laboratory Winnipeg, Winnipeg, Manitoba


    Stock #: JFS14520J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14520J

    ASTM International
    is a member of CrossRef.

    Author
    Title Removal of a PCR Inhibitor and Resolution of DNA STR Types in Mixed Human-Canine Stains from a Five Year Old Case
    Symposium , 0000-00-00
    Committee E30