Volume 44, Issue 2 (March 1999)

    Preparation of Carboxyhemoglobin Standards and Calculation of Spectrophotometric Quantitation Constants

    (Received 7 April 1998; accepted 13 July 1998)

    CODEN: JFSOAD

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    Abstract

    A method was developed for the preparation of carboxyhemoglobin (COHB) standards, which were stable for more than four months with the prepared control remaining within acceptable limits during this time. A mathematical equation was developed to more accurately determine the constants A and B used in the equation COHB% = 100[(CB)/(AB)]; where B = 0% COHB peak ratio at 540 nm and 579 nm; A = 100% COHB peak ratio at 540 nm and 579 nm; and C = the peak ratio at 540 nm and 579 nm for the blood being analyzed. The following equations were developed to calculate A and B: B = Pavg − (P)[(PavgNavg)/(PN)]; A = B + (PavgNavg)/(PN), Pavg = average peak ratio 540/579 for the positive standard run on the spectrophotometer; P = average decimal concentration measured on the CO-OXIMETER for the positive standard; Navg = average peak ratio 540/579 for the negative standard; N = average decimal concentration measured on the CO-OXIMETER for the negative standard. The new equations provided results consistent with those obtained from a CO-OXIMETER.


    Author Information:

    Chaturvedi, AK
    Supervisory research chemist, medical technologist, and research chemists, Civil Aeromedical Institute, Federal Aviation Administration, Toxicology and Accident Research Laboratory, Oklahoma City, OK

    Canfield, DV
    Supervisory research chemist, medical technologist, and research chemists, Civil Aeromedical Institute, Federal Aviation Administration, Toxicology and Accident Research Laboratory, Oklahoma City, OK

    Smith, M
    Supervisory research chemist, medical technologist, and research chemists, Civil Aeromedical Institute, Federal Aviation Administration, Toxicology and Accident Research Laboratory, Oklahoma City, OK

    Ritter, RM
    Supervisory research chemist, medical technologist, and research chemists, Civil Aeromedical Institute, Federal Aviation Administration, Toxicology and Accident Research Laboratory, Oklahoma City, OK


    Stock #: JFS14475J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14475J

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    Author
    Title Preparation of Carboxyhemoglobin Standards and Calculation of Spectrophotometric Quantitation Constants
    Symposium , 0000-00-00
    Committee E30