ISSN: 0022-1198
CODEN: JFSCA
Page Count: 5

The Determination of Lysergide (LSD) in Urine by High-Performance Liquid Chromatography-Isotope Dilution Mass Spectrometry (IDMS)
Kidd, AS
Biomedical Science Division, Kings College London,

Webb, KS
Laboratory of the Government Chemist, TW

White, SA
Laboratory of the Government Chemist, TW

(Received 2 June 1998; accepted 17 August 1998)

Abstract

The use of isotope dilution mass spectrometry (IDMS) has been investigated for the forensic confirmation of lysergic acid diethylamide (LSD) in urine by LC-MS. The advantages of using a deuterated analog of LSD as an internal standard over methysergide are discussed. This study includes a comparison of the electrospray mass spectra of LSD, LSD-d³ and methysergide, and discusses the choice of suitable ions for use in selected ion monitoring (SIM) mode. An IDMS method is presented for the LC-MS confirmation of LSD in urine, with a limit of quantification (LOQ) of 0.5 ng/mL, reflecting the forensic requirement at this laboratory. Under some circumstances the LOQ can be improved to 0.1 ng/mL. This method is linear in the range tested (up to 10 ng/mL LSD in urine) and has been validated in terms of accuracy and precision.

Keywords:
forensic science, forensic toxicology, lysergic acid diethylamide, urine, high performance liquid chromatography-mass spectrometry, electrospray ionization, isotope dilution mass spectrometry

Paper ID: JFS14467J
DOI: 10.1520/JFS14467J
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