Volume 44, Issue 2 (March 1999)

    The Determination of Lysergide (LSD) in Urine by High-Performance Liquid Chromatography-Isotope Dilution Mass Spectrometry (IDMS)

    (Received 2 June 1998; accepted 17 August 1998)

    CODEN: JFSOAD

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    Abstract

    The use of isotope dilution mass spectrometry (IDMS) has been investigated for the forensic confirmation of lysergic acid diethylamide (LSD) in urine by LC-MS. The advantages of using a deuterated analog of LSD as an internal standard over methysergide are discussed. This study includes a comparison of the electrospray mass spectra of LSD, LSD-d3 and methysergide, and discusses the choice of suitable ions for use in selected ion monitoring (SIM) mode. An IDMS method is presented for the LC-MS confirmation of LSD in urine, with a limit of quantification (LOQ) of 0.5 ng/mL, reflecting the forensic requirement at this laboratory. Under some circumstances the LOQ can be improved to 0.1 ng/mL. This method is linear in the range tested (up to 10 ng/mL LSD in urine) and has been validated in terms of accuracy and precision.


    Author Information:

    Kidd, AS
    Biomedical Science Division, Kings College London, Strand, London,

    Webb, KS
    Laboratory of the Government Chemist, Teddington Middlesex, TW

    White, SA
    Laboratory of the Government Chemist, Teddington Middlesex, TW


    Stock #: JFS14467J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14467J

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    Author
    Title The Determination of Lysergide (LSD) in Urine by High-Performance Liquid Chromatography-Isotope Dilution Mass Spectrometry (IDMS)
    Symposium , 0000-00-00
    Committee E30