Determination of Lysergic Acid Diethylamide in Body Fluids by High-Performance Liquid Chromatography and Fluorescence Detection—A More Sensitive Method Suitable for Routine Use

    Volume 44, Issue 2 (March 1999)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 3


    Geier, A
    Institut für Rechtsmedizin der Universität München, München,

    Bergemann, D
    Institut für Rechtsmedizin der Universität München, München,

    von Meyer, L
    Institut für Rechtsmedizin der Universität München, München,

    (Received 13 May 1998; accepted 27 July 1998)

    Abstract

    A new method for determination of lysergic acid diethylamide (LSD) in body fluids by high-performance liquid chromatography and fluorescence detection was developed based on previously published methods. The new method is suitable for confirmation of samples tested positive by immunoassay, avoiding loss of LSD by absorption to surfaces. The reduced loss of LSD results in improved sensitivity. This is achieved by adding ethylene glycol to the samples, which cover glass surfaces. This principle can similarly be used to improve analysis of other drugs.

    Body fluids for analysis included urine and whole blood. An internal standard was applied for quantification of LSD.

    The new method offers satisfying precision data and has a detection limit of less than 0.05 ng/nL.


    Paper ID: JFS14466J

    DOI: 10.1520/JFS14466J

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    Title Determination of Lysergic Acid Diethylamide in Body Fluids by High-Performance Liquid Chromatography and Fluorescence Detection—A More Sensitive Method Suitable for Routine Use
    Symposium , 0000-00-00
    Committee E30