Volume 44, Issue 1 (January 1999)

    Deoxyribonuclease I Phenotyping from Saliva Stains

    (Received 3 March 1998; accepted 25 May 1998)


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    Good typing results were obtained using a newly developed method for extraction and purification of deoxyribonuclease I (DNase I) from saliva stains. Previously, DNase I phenotyping from saliva stains has been unsuccessful because of low enzyme activity and heavy contamination. Salivary DNase I was extracted from stains using phosphate buffer containing Nonidet P-40. Extracts were purified using Phenyl Sepharose CL-4B gel. Electrophoresis was performed, and DNase I was successfully phenotyped. All of the DNase I phenotypes, which were obtained from saliva stains using this new method, were identical to the phenotypes determined from urine samples. Moreover, DNase I was correctly phenotyped from saliva stains that had been stored for over three months at room temperature or at 37°C. These results suggest that DNase I polymorphisms provide valuable information for forensic characterization of saliva stains.

    Author Information:

    Tsubota, E
    Fukui Medical University, Fukui,

    Kishi, K
    Gunma University School of Medicine, Gunma,

    Sawazaki, K

    Aoyama, M
    Fukui Medical University, Fukui,

    Iida, R
    Fukui Medical University, Fukui,

    Matsuki, T
    Fukui Medical University, Fukui,

    Yasuda, T
    Gunma University School of Medicine, Gunma,

    Stock #: JFS14428J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14428J

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    Title Deoxyribonuclease I Phenotyping from Saliva Stains
    Symposium , 0000-00-00
    Committee E30