Journal Published Online: 01 January 1999
Volume 44, Issue 1

Allele Distribution at Nine STR Loci—D3S1358, vWA, FGA, TH01, TPOX, CSF1PO, D5S818, D13S317 and D7S820—in the Japanese Population by Multiplex PCR and Capillary Electrophoresis

CODEN: JFSCAS

Abstract

Nine tetranucleotide short tandem repeat (STR) loci, D3S1358, vWA, FGA TH01, TPOX, CSF1PO, D5S818, D13S317 and D7S820, were analyzed in the Japanese population with a newly released kit for personal identification using multiplex PCR with fluorescent-labeled primers following capillary electrophoresis. The observed heterozygosities were 0.67, 0.77, 0.82, 0.61, 0.62, 0.73, 0.78, 0.81 and 0.74, respectively, and the combined discrimination power of the nineplex was 0.9999999991. None of the nine loci deviated from Hardy-Weinberg equilibrium expectations using the chi-square test, homozygosity test, likelihood ratio test and exact test after the grouping of the alleles. The nine STR loci allele frequencies were significantly different from those of other ethnic populations.

Author Information

Yamamoto, T
Nagoya University School of Medicine, Nagoya, Japan
Uchihi, R
Nagoya University School of Medicine, Nagoya, Japan
Nozawa, H
Nagoya University School of Medicine, Nagoya, Japan
Huang, X-L
Nagoya University School of Medicine, Nagoya, Japan
Leong, Y-K
Nagoya University School of Medicine, Nagoya, Japan
Tanaka, M
Nagoya University School of Medicine, Nagoya, Japan
Mizutani, M
Nagoya University School of Medicine, Nagoya, Japan
Tamaki, K
Nagoya University School of Medicine, Nagoya, Japan
Katsumata, Y
Nagoya University School of Medicine, Nagoya, Japan
Pages: 4
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Stock #: JFS14426J
ISSN: 0022-1198
DOI: 10.1520/JFS14426J