Volume 44, Issue 1 (January 1999)

    Allele Distribution at Nine STR Loci—D3S1358, vWA, FGA, TH01, TPOX, CSF1PO, D5S818, D13S317 and D7S820—in the Japanese Population by Multiplex PCR and Capillary Electrophoresis

    (Received 16 April 1998; accepted 18 June 1998)

    CODEN: JFSOAD

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    Abstract

    Nine tetranucleotide short tandem repeat (STR) loci, D3S1358, vWA, FGA TH01, TPOX, CSF1PO, D5S818, D13S317 and D7S820, were analyzed in the Japanese population with a newly released kit for personal identification using multiplex PCR with fluorescent-labeled primers following capillary electrophoresis. The observed heterozygosities were 0.67, 0.77, 0.82, 0.61, 0.62, 0.73, 0.78, 0.81 and 0.74, respectively, and the combined discrimination power of the nineplex was 0.9999999991. None of the nine loci deviated from Hardy-Weinberg equilibrium expectations using the chi-square test, homozygosity test, likelihood ratio test and exact test after the grouping of the alleles. The nine STR loci allele frequencies were significantly different from those of other ethnic populations.


    Author Information:

    Nozawa, H
    Associate Professor, Assistant Professor, Postgraduate Students, Associate Professor and Professor, Nagoya University School of Medicine, Nagoya,

    Tamaki, K
    Associate Professor, Assistant Professor, Postgraduate Students, Associate Professor and Professor, Nagoya University School of Medicine, Nagoya,

    Mizutani, M
    Associate Professor, Assistant Professor, Postgraduate Students, Associate Professor and Professor, Nagoya University School of Medicine, Nagoya,

    Tanaka, M
    Associate Professor, Assistant Professor, Postgraduate Students, Associate Professor and Professor, Nagoya University School of Medicine, Nagoya,

    Uchihi, R
    Associate Professor, Assistant Professor, Postgraduate Students, Associate Professor and Professor, Nagoya University School of Medicine, Nagoya,

    Yamamoto, T
    Associate Professor, Assistant Professor, Postgraduate Students, Associate Professor and Professor, Nagoya University School of Medicine, Nagoya,

    Huang, X-L
    Associate Professor, Assistant Professor, Postgraduate Students, Associate Professor and Professor, Nagoya University School of Medicine, Nagoya,

    Leong, Y-K
    Associate Professor, Assistant Professor, Postgraduate Students, Associate Professor and Professor, Nagoya University School of Medicine, Nagoya,

    Katsumata, Y
    Associate Professor, Assistant Professor, Postgraduate Students, Associate Professor and Professor, Nagoya University School of Medicine, Nagoya,


    Stock #: JFS14426J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14426J

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    Author
    Title Allele Distribution at Nine STR Loci—D3S1358, vWA, FGA, TH01, TPOX, CSF1PO, D5S818, D13S317 and D7S820—in the Japanese Population by Multiplex PCR and Capillary Electrophoresis
    Symposium , 0000-00-00
    Committee E30