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    Volume 44, Issue 1 (January 1999)

    Blood Grouping of Mixed Bloodstains Using Immunocytochemical Methods

    (Received 21 January 1998; accepted 1 June 1998)

    CODEN: JFSOAD

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    Abstract

    Immunocytochemical methods to determine the ABO blood group of each blood of mixed bloodstains have been developed. Mixed bloodstains were made on surgical blades and a cedar board. The blades were dipped into blood and then dipped into blood of a different group at intervals of 30, 20, 15, 10 and 5 s. Two drops of blood were dropped on a cedar board and then two drops of blood of a different group were dropped there at the same intervals. The bloodstains were dried for a week. The blood samples were removed from the blades or the cedar board and processed according with a routine histological method. Three serial thin sections were obtained. After deparaffinization, the sections were treated in papain solution for 2 h at 36°C, to unmask antigenic sites on red cell membranes. The labeled streptavidin-biotin (LSAB) and peroxidase-anti-peroxidase (PAP) methods were used to detect A and B antigens, and an indirect immunocytochemical method for H antigen. These immunocytochemical methods showed specific immunologic reactions and allowed determination of the blood group of each blood of mixed bloodstains. Further, these methods indicated a possibility to determine who was stabbed first, in cases where two or more victims were stabbed with a single knife.


    Author Information:

    Nagai, A
    Gifu University School of Medicine, Gifu,

    Nakamura, I
    Gifu University School of Medicine, Gifu,

    Ohya, I
    Gifu University School of Medicine, Gifu,

    Yamada, S
    Gifu University School of Medicine, Gifu,

    Takayama, T
    Gifu University School of Medicine, Gifu,

    Bunai, Y
    Gifu University School of Medicine, Gifu,

    Watanabe, Y
    Gifu University School of Medicine, Gifu,


    Stock #: JFS14418J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14418J

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    Title Blood Grouping of Mixed Bloodstains Using Immunocytochemical Methods
    Symposium , 0000-00-00
    Committee E30