ISSN: 0022-1198
CODEN: JFSCA
Page Count: 13

Development and Population Study of an Eight-Locus Short Tandem Repeat (STR) Multiplex System
Lins, AM
Director, Research and Development, DNA Typing Promega Corporation, WI

Sprecher, CJ
Director, Research and Development, DNA Typing Promega Corporation, WI

Rabbach, DR
Director, Research and Development, DNA Typing Promega Corporation, WI

Taylor, JA
Director, Research and Development, DNA Typing Promega Corporation, WI

Bacher, JW
Director, Research and Development, DNA Typing Promega Corporation, WI

Schumm, JW
Director, Research and Development, DNA Typing Promega Corporation, WI

Micka, KA
Director, Research and Development, DNA Typing Promega Corporation, WI

Bever, RA
BODE Technologies, VA

Creacy, SD
Creacy Enterprises, Inc., NC

(Received 16 February 1998; accepted 19 February 1998)

Abstract

Amplification of short tandem repeat (STR) loci has become a useful tool for human identification applications. To improve throughput and efficiency for such uses, the polymorphic STR loci CSF1PO, TPOX, TH01, vWA, D16S539, D7S820, D13S317, D5S818, F13A01, FESFPS, F13B, and LPL have been evaluated, developed, and configured into fluorescently labeled multiplex systems. Eight of these STR loci were combined to generate the PowerPlex™ System, a two-color multiplex system that supports rapid, accurate, reliable analysis and designation of alleles. The remaining four loci comprise the FFFL System, a one-color multiplex system. The PowerPlex™ System may be evaluated alternatively as two one-color, four-locus multiplex systems. CTTv Multiplex and GammaSTR™ Multiplex. The products of multiplex amplification may be analyzed with a variety of fluorescence detection instruments. Determination of genotypes of over 200 individuals from each of three different population/ethnic groups revealed independence of inheritance of the loci and allowed calculation of matching probability, typical paternity index, and power of exclusion for each multiplex.

Keywords:
forensic science, DNA typing, short tandem repeats, multiplex, polymerase chain reaction, CSF1PO, TPOX, TH01, vWA, D16S539, D7S820, D13S317, D5S818, F13A01, FESFPS, F13B, LPL, PowerPlex

Paper ID: JFS14381J
DOI: 10.1520/JFS14381J
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