Volume 43, Issue 5 (September 1998)

    Development of a Highly Polymorphic STR Marker for Identity Testing Purposes at the Human Androgen Receptor Gene (HUMARA)

    (Received 22 September 1997; accepted 29 January 1998)

    CODEN: JFSOAD

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    Abstract

    We developed a non-isotopic method which improves the technical quality of the X-linked HUMARA locus typing process. The use of formamide and a low concentration of acrylamide increased resolution and sharpness of HUMARA alleles in silver-stained polyacrylamide gels. In addition, the construction of an allelic ladder containing amplified sequence of 9 alleles (even-numbered alleles) of the HUMARA locus, allows confident, rapid and precise assigment of discretely defined alleles. Allele and genotype frequencies for the HUMARA locus were determined in a French Canadian population sample. Observed genotype frequencies in females conformed to Hardy-Weinberg expectations. Furthermore, the HUMARA locus is highly polymorphic with 18 observed alleles and an heterozygosity value of 89.3%. Also, this locus has average powers of discrimination of 97.8% and 88.7% for testing samples of female and male origin, respectively. In the French Canadian population, the average probability of excluding a random man as the father in paternity analysis when both mother and daughter are tested for this locus is 88.0%. Together, the results indicate that the HUMARA locus provides a highly discriminatory system that is appropriate for the purposes of forensic identification and paternity testing involving a female child.


    Author Information:

    Perreault, C
    Centre de Recherche Guy Bernier, Hopital Maisonneuve-Rosemont, Montreal, Quebec

    Desmarais, D
    PRO-ADN Diagnostic, Polyservices Maisonneuve-Rosemont, Montreal, Quebec

    Busque, L

    Chakraborty, R
    Human Genetics Center, University of Texas, Houston, TX

    Zhong, Y
    Human Genetics Center, University of Texas, Houston, TX


    Stock #: JFS14355J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14355J

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    Author
    Title Development of a Highly Polymorphic STR Marker for Identity Testing Purposes at the Human Androgen Receptor Gene (HUMARA)
    Symposium , 0000-00-00
    Committee E30