Volume 43, Issue 5 (September 1998)

    Evaluation of Aspartic Acid Racemization Ratios in the Human Femur for Age Estimation

    (Received 11 June 1997; accepted 30 December 1997)

    CODEN: JFSOAD

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    Abstract

    Levels of D-aspartic acid (D/L ratio) in cranial noncollagen proteins (acid-soluble peptide fractions) have been reported to increase with age. We isolated total amino acid fractions from the femur and separately isolated acid-insoluble collagen fraction and acid-soluble peptide fractions; then D/L ratios were measured from each fraction by gas chromatography. We evaluated the applicability of their D/L ratios for age estimation based on their correlation coefficient. A sex-related difference was observed in the D/L ratio. In particular, aged females showed a low ratio, suggesting an association with bone disorders. In males, the D/L ratios of acid-soluble peptide fraction showed the highest correlation rate (r = 0.969) with age, and those of total amino acid fraction showed the highest correlation rate (r = 0.633) with age in females. Without separation of male and female, the D/L ratios of total amino acid fraction showed the highest value (r = 0.853). The D/L ratio of acid-soluble peptide fractions differed according to the size of bone powder particles, being higher for larger particle sizes. These results suggest that the application of D/L ratio from total amino acid fraction is the most effective method for estimating age using the human femur. However, care is necessary when studing cadavers that might be females.


    Author Information:

    Kishi, K
    Department of Laboratory Animal Science and Department of Clinical Pathology, Saitama Cancer Center Research Institute, Saitama,

    Ohtani, S
    Kanagawa Dental College, Yokosuka, Kanagawa,

    Kobayashi, Y
    Department of Laboratory Animal Science and Department of Clinical Pathology, Saitama Cancer Center Research Institute, Saitama,

    Matsushima, Y
    Department of Laboratory Animal Science and Department of Clinical Pathology, Saitama Cancer Center Research Institute, Saitama,


    Stock #: JFS14339J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14339J

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    Title Evaluation of Aspartic Acid Racemization Ratios in the Human Femur for Age Estimation
    Symposium , 0000-00-00
    Committee E30