Dandruff as a Potential Source of DNA in Forensic Casework

    Volume 43, Issue 4 (July 1998)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 2


    Budowle, B
    Forensic Science Research Unit, FBI Academy, Quantico, VA

    Entrala, C
    University of Granada, Granada,

    Villanueva, E
    University of Granada, Granada,

    Lorente, JA
    University of Granada, Granada,

    Alvarez, JC
    University of Granada, Granada,

    Lorente, M
    University of Granada, Granada,

    (Received 12 August 1997; accepted 8 December 1997)

    Abstract

    Dandruff is a clinical alteration of the skin that consists histologically of orthokeratotic clumps with minute parakeratotic foci found in inflammatory pathologies such as seborrheic dermatitis and psoriasis. Therefore, some nucleated cells should be found in dandruff and hence there is a possibility that forensically typeable DNA could be extracted from dandruff.

    Because of a particular case in which we were involved, a study was carried out to determine whether or not DNA could be extracted from dandruff, and if the two most widely used extraction techniques (Chelex and organic) would be applicable.

    Results show that sufficient quantities of DNA (more than 30 to 40 ng) can be obtained from as little as 1.0 to 1.5 mg of dandruff. Both methods yield DNA, although the organic procedure seems to yield more (72.5 ng Chelex vs. 183.3 ng organic). All the DNA samples extracted were typed correctly for the loci HUMTH01 and HUMvWA.

    Therefore, dandruff can be considered a potential source of DNA for forensic identification


    Paper ID: JFS14326J

    DOI: 10.1520/JFS14326J

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    Title Dandruff as a Potential Source of DNA in Forensic Casework
    Symposium , 0000-00-00
    Committee E30