Journal Published Online: 01 July 1998
Volume 43, Issue 4

Polymorphism and Sequence Variations of the HumCD4 Pentameric Microsatellite in an Italian Population Sample

CODEN: JFSCAS

Abstract

A sample of 265 subjects from central Italy was analyzed at the HumCD4 locus by polymerase-chain-reaction (PCR). Phenotypes were identified by comparison with a sequenced ladder, after high-resolution horizontal polyacrylamide gel electrophoresis (PAGE) followed by silver staining. A set of representative alleles was sequenced by Taq-cycle-sequencing with dye terminator labeling and capillary gel electrophoresis strategies. Eight common alleles—5,6,7,8,9,10,11,12—and a rare larger 14, never before described in Caucasians, were found. Allele and genotype frequencies were similar to those described in former studies on Caucasians, with a prevalency of alleles number 5, 6, 10. Sequence analysis showed that the polymorphism is due to a pentameric TTTTC basic motif, tandemly repeated, and that from allele number 10 onwards the fourth repeat presents a T to C translation (CTTTC). Instead, allele number 9 may exist in two forms, because 75% of alleles examined in this study presented the CTTTC motif at the fourth position, while the remaining 25% had the basic repeat structure.

Author Information

Tagliabracci, A
Istituto di Medicina Legale, Università degli Studi di Ancona, Policlinico Torrette, Ancona, Italy
Buscemi, L
Istituto di Medicina Legale, Università degli Studi di Ancona, Policlinico Torrette, Ancona, Italy
Bianchi, F
Istituto di Medicina Legale, Università degli Studi di Ancona, Policlinico Torrette, Ancona, Italy
Sassaroli, C
Istituto di Medicina Legale, Università degli Studi di Ancona, Policlinico Torrette, Ancona, Italy
Ricci, U
Servizio di Genetica Umana, Azienda Meyer, Università di Firenze, Firenze, Italy
Neri, TM
Cattedra di Genetica Medica, Università di Parma, Ospedale Maggiore, Parma, Italy
Rodriguez, D
Istituto di Medicina Legale, Università degli Studi di Ancona, Policlinico Torrette, Ancona, Italy
Pages: 4
Price: $25.00
Related
Reprints and Permissions
Reprints and copyright permissions can be requested through the
Copyright Clearance Center
Details
Stock #: JFS14316J
ISSN: 0022-1198
DOI: 10.1520/JFS14316J