ISSN: 0022-1198
CODEN: JFSCA
Page Count: 5

Simultaneous Determination of STR Polymorphism and a New Nucleotide Substitution in Its Flanking Region at the CD4 Locus
Watanabe, G
Postgraduate student, associate professor, and professor, Yamagata University School of Medicine,

Yuasa, I
Assistant professor, Tottori University School of Medicine,

Umetsu, K
Postgraduate student, associate professor, and professor, Yamagata University School of Medicine,

Suzuki, T
Postgraduate student, associate professor, and professor, Yamagata University School of Medicine,

(Received 29 May 1997; accepted 8 December 1997)

Abstract

In the course of the investigation of a pentanucleotide repeat polymorphism at the human CD4 locus, a C-A transversion was found at the position corresponding to the 3′ end of the original forward primer presented by Edwards et al. (1). In the present study, the simultaneous determination of the new sequence polymorphism and the pentanucleotide repeat polymorphism at the CD4 locus was attempted. To achieve this purpose, we adopted amplified product length polymorphism (APLP) analysis and designed some new allele-specific forward primers tagged with non-complementary nucleotides differing in length. A total of 646 DNA samples from peripheral blood of Japanese, Chinese and German populations were investigated. Although the C-A transversion was restricted to CD4*5, a new subtype allele with A and 5 repeats, designated CD4*5A, was observed at polymorphic frequencies in the three populations. The simultaneous genotyping by APLP analysis resulted in dramatically increased heterozygosity and discriminating power of the human CD4 locus.

Keywords:
forensic science, DNA typing, short tandem repeat, CD4 locus, population study, C-A transversion, pentanucleotide repeat

Paper ID: JFS14298J
DOI: 10.1520/JFS14298J
ASTM International is a member of CrossRef.