Volume 42, Issue 6 (November 1997)

    Analysis of Allele Distribution for Six Short Tandem Repeat Loci in the French Canadian Population of Québec

    (Received 26 November 1996; accepted 14 February 1997)

    CODEN: JFSOAD

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    Abstract

    Short tandem repeat (STR) loci represent a rich source of highly polymorphic markers in the human genome which are useful for the purposes of forensic identification and determination of biological relatedness of individuals. Here, as a part of an ongoing extensive study, we report the analysis of a multilocus genotype survey of 642 to 870 chromosomes in the French Canadian Caucasian population of Québec at six STR loci. The loci HUMCS-FIPO, HUMTPOX, HUMTH01, HUMF13A01, HUMFESFPS, and HUMvWA were typed using two multiplex polymerase chain reactions (PCR). Amplified DNA samples were subsequently analyzed by polyacrylamide gel electrophoresis followed by silver staining. The heterozygote frequencies of the loci range from 0.614 to 0.820 (0.661 to 0.818 expected) and the number of alleles from 7 to 12 per locus. Although statistically significant deviation from Hardy-Weinberg expectations of genotype frequencies was noted at some loci by one or more tests, in general, the genotype frequencies are well estimated from the product of allele frequencies at all loci. The most frequent six-locus genotype is expected to occur in the French Canadian population with a frequency of 3.50 by 10−5 and together, these six loci have an average probability of discrimination of 0.9999985. The study presented here indicates that these six STR loci are informative genetic markers for identity testing purposes in the French Canadian Caucasian population of Québec.


    Author Information:

    Desmarais, D
    Centre de Recherche Guy Bernier, Hôpital Maisonnetive-Rosemont, Montréal, Québec

    Schumm, JW
    Promega Corporation, Madison, WI

    Busque, L
    Centre de Recherche Guy Bernier, Hôpital Maisonnetive-Rosemont, Montréal, Québec

    Chakraborty, R
    Human Genetics Center, University of Texas, Houston, TX

    Provost, S
    Centre de Recherche Guy Bernier, Hôpital Maisonnetive-Rosemont, Montréal, Québec

    Zhong, Y
    Human Genetics Center, University of Texas, Houston, TX


    Stock #: JFS14276J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14276J

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    Author
    Title Analysis of Allele Distribution for Six Short Tandem Repeat Loci in the French Canadian Population of Québec
    Symposium , 0000-00-00
    Committee E30