Genetic Individualization of Domestic Cats Using Feline STR Loci for Forensic Applications

    Volume 42, Issue 6 (November 1997)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 13


    Stephens, JC
    Laboratory of Genomic Diversity, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD

    Lyons, LA
    Laboratory of Genomic Diversity, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD

    Menotti-Raymond, M
    Laboratory of Genomic Diversity, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD

    O'Brien, SJ
    Laboratory of Genomic Diversity, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD

    David, VA
    Intramural Research Support Program, SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD

    (Received 1 October 1996; accepted 28 February 1997)

    Abstract

    A group of ten short tandem repeat (STR) loci suitable for PCR typing from DNA of domestic cats is evaluated for genetic individualization using blinded samples of eight putative feline blood specimens. The ten loci were also typed in a 70 member cat pedigree to demonstrate Mendelian inheritance and independent assortment. A “match window” or measurement precision estimate was empirically established by determining the maximum gel migration difference among alleles identical by descent in different individuals of the pedigree. Hardy-Weinberg equilibrium and abundant heterozygosity was observed for each locus in cat population samples from Canada and the USA. The probabilities of two unrelated individuals matching by chance (Pm) at all ten loci was estimated as 1.35 × 10−10. We present a conservative approach to compute, for forensic consideration, the mathematical likelihood of a chance genotypic match between DNA evidence from a crime scene and the suspect composite STR genotypes for species or populations when genotype frequency information is not available.


    Paper ID: JFS14258J

    DOI: 10.1520/JFS14258J

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    Author
    Title Genetic Individualization of Domestic Cats Using Feline STR Loci for Forensic Applications
    Symposium , 0000-00-00
    Committee E30