Volume 42, Issue 6 (November 1997)

    Extraction of Single-Copy Nuclear DNA from Forensic Specimens with a Variety of Postmortem Histories

    (Received 26 September 1996; accepted 21 March 1997)

    CODEN: JFSOAD

      Format Pages Price  
    PDF Version 7 $25   ADD TO CART


    Abstract

    Specimens of human bone, teeth and dried blood spots from 3 months to 91 years old, with a variety of postmortem histories, were used in a comparative study of recovery of single-copy nuclear DNA sequences from forensic material. Sequences of the amelogenin and HLA-DPB1 genes were chosen for their value in sexing and identification. Sequences of the mitochondrial non-coding region V were also amplified to compare the recovery of mitochondrial and single-copy nuclear DNA. A variation of the silica method for DNA extraction was refined for application to the forensic specimens in this sample. Single-copy nuclear DNA was amplified from 100% of recent postoperative bone specimens (n = 6), 80% of forensic teeth and bone specimens (n = 10), 78% of recently extracted teeth (n = 18), 78% of exhumed bone up to 91 years old (n = 37) and 69% of 15 year old bone specimens fixed in 10% formalin (n = 20). Amelogenin sexing was correct in 85% of cases (n = 74) in which the sex of the donor had been recorded. There was no correlation between the age of the specimen and the extent of DNA preservation.


    Author Information:

    Chamberlain, AT
    University of Sheffield, Sheffield,

    Smillie, DM
    Trent Centre, National Blood Service, Longley Lane, Sheffield,

    Evison, MP
    University of Sheffield, Sheffield,


    Stock #: JFS14257J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14257J

    ASTM International
    is a member of CrossRef.

    Author
    Title Extraction of Single-Copy Nuclear DNA from Forensic Specimens with a Variety of Postmortem Histories
    Symposium , 0000-00-00
    Committee E30