Volume 42, Issue 5 (September 1997)

    Sequential Multiplex Amplification: Utility in Forensic Casework with Minimal Amounts of DNA and Partially Degraded Samples

    (Received 5 September 1996; accepted 7 February 1997)

    CODEN: JFSOAD

      Format Pages Price  
    PDF 3 $25   ADD TO CART


    Abstract

    Since its introduction, PCR has become a widely-used, routine technique in forensic laboratories. A number of PCR protocols that were developed originally are now being replaced by more powerful approaches, particularly those based on multiplex amplification of short tandem repeat (STR) loci.

    One alternative form of multiplex PCR amplification, called Sequential Multiplex Amplification (SMA), was designed to amplify a single locus and then recover and reuse the remaining genomic DNA as a template for subsequent PCR. The SMA process could be repeated several times. SMA has proven to be useful in typing genomic DNA contained in stored PCR samples and analyzing samples of limited quality and/or quantity for multiple loci. The efficacy of the use of SMA for actual typing of casework samples permitted typing for a second locus 98.11% of the samples considered; 70.75% were typeable for a third locus, and 16.98% for a fourth locus.


    Author Information:

    Budowle, B
    FSRTC—Laboratory Division—FBI Academy, Quantico, VA

    Villanueva, E
    Facultad de Medicina Universidad de Granada, Granada,

    Lorente, JA
    Facultad de Medicina Universidad de Granada, Granada,

    Carlos Alvarez, J
    Facultad de Medicina Universidad de Granada, Granada,

    Wilson, MR
    FSRTC—Laboratory Division—FBI Academy, Quantico, VA

    Lorente, M
    Facultad de Medicina Universidad de Granada, Granada,


    Stock #: JFS14232J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14232J

    ASTM International
    is a member of CrossRef.

    Author
    Title Sequential Multiplex Amplification: Utility in Forensic Casework with Minimal Amounts of DNA and Partially Degraded Samples
    Symposium , 0000-00-00
    Committee E30