Volume 42, Issue 5 (September 1997)

    HumCD4—Validation of a STR System for Forensic Purposes in an Austrian Caucasian Population Sample

    (Received 1 April 1996; accepted 31 December 1996)

    CODEN: JFSOAD

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    Abstract

    The short tandem repeat system HumCD4 was amplified by the polymerase chain reaction (PCR) on blood samples from 304 unrelated Austrian Caucasians and analyzed by horizontal, non-denaturing polyacrylamide electrophoresis. The mean exclusion chance was 0.417, the discriminating power 0.850 and the heterozygosity rate 0.628. The observed phenotype distribution is in Hardy-Weinberg equilibrium. In 100 families (200 meioses) no mutations were found. Sufficient amplification could be achieved with as little as 80 pg of high molecular weight cell-line DNA. In a degradation experiment DNA extracted from bloodstains stored for up to 28 days in a moist chamber and DNA boiled for up to 18 min could be amplified. A duplex PCR with TH01 is proposed.


    Author Information:

    Klintschar, M
    Manager of the Forensic DNA Laboratory and physician, Institute for Legal Medicine, University, Graz,

    Crevenna, R
    Manager of the Forensic DNA Laboratory and physician, Institute for Legal Medicine, University, Graz,


    Stock #: JFS14228J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14228J

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    Author
    Title HumCD4—Validation of a STR System for Forensic Purposes in an Austrian Caucasian Population Sample
    Symposium , 0000-00-00
    Committee E30