Products of Partial Digestion with <i>Hae</i> III. Part 1. Characterization, Casework Experience and Confirmation of the Theory of Three , Four and Five Banded RFLP Pattern Origins Using Partial Digestion

Volume 42, Issue 5 (September 1997)

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ISSN: 0022-1198
CODEN: JFSCA
Page Count: 14

Products of Partial Digestion with Hae III. Part 1. Characterization, Casework Experience and Confirmation of the Theory of Three-, Four- and Five-Banded RFLP Pattern Origins Using Partial Digestion
Riech, AK
Ohio Bureau of Criminal Identification and Investigation, OH

Duewer, DL
Chemical Sciences and Technology Laboratory, National Institute of Standards and Technology, MD

Benzinger, EA
Ohio Bureau of Criminal Identification and Investigation, OH

Emerek, EA
Ohio Bureau of Criminal Identification and Investigation, OH

Deadman, H
DNA Unit, Room 3905, Federal Bureau of Investigation, DC

Thompson, JL
Ohio Bureau of Criminal Identification and Investigation, OH

Lovekamp, ML
Ohio Bureau of Criminal Identification and Investigation, OH

Grigsby, NL
Ohio Bureau of Criminal Identification and Investigation, OH

Sallee, PJ
Ohio Bureau of Criminal Identification and Investigation, OH

(Received 9 August 1996; accepted 24 January 1997)

Abstract

The sizes of Hae III partial digestion products at D1S7, D2S44, D4S139, D5S110, D10S28, and D17S26 were evaluated in experimentally generated partial digestions of liquid blood DNA. The partial digestion products were highly predictable, suggesting a very high level of sequence conservation in regions flanking variable number tandem repeat (VNTR) blocks. Partial digestion bands associated with three-or-more-banded patterns were also characterized. Partial digestion of three-banded patterns can be used to determine whether the extra bands arise due to internal Hae III sites in the VNTR block and to identify hidden three-banded patterns. Partial digestion products from forensic casework also conformed to size expectations. Presumed partial digestion bands from 27 forensic samples were compared to the experimentally generated data. The causes of partial digestion are examined and recommendations for interpreting forensic DNA evidence exhibiting partial digestion products are given.

Keywords:
forensic science, band shift, band sizing, DNA typing, gel electrophoresis, restriction fragment length polymorphism, variable number tandem repeat, partial digestion

Paper ID: JFS14220J
DOI: 10.1520/JFS14220J
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Author Title Products of Partial Digestion with Hae III. Part 1. Characterization, Casework Experience and Confirmation of the Theory of Three-, Four- and Five-Banded RFLP Pattern Origins Using Partial Digestion Symposium , 0000-00-00 Committee E30

		SEDL / Journals / Journal of Forensic Sciences (JOFS) / Citation Page Volume 42, Issue 5 (September 1997) Click here to download this paper now for $25 PDF (848K) View License Agreement ISSN: 0022-1198 CODEN: JFSCA Page Count: 14 *Products of Partial Digestion with Hae* III. Part 1. Characterization, Casework Experience and Confirmation of the Theory of Three-, Four- and Five-Banded RFLP Pattern Origins Using Partial Digestion** Riech, AK Ohio Bureau of Criminal Identification and Investigation, OH Duewer, DL Chemical Sciences and Technology Laboratory, National Institute of Standards and Technology, MD Benzinger, EA Ohio Bureau of Criminal Identification and Investigation, OH Emerek, EA Ohio Bureau of Criminal Identification and Investigation, OH Deadman, H DNA Unit, Room 3905, Federal Bureau of Investigation, DC Thompson, JL Ohio Bureau of Criminal Identification and Investigation, OH Lovekamp, ML Ohio Bureau of Criminal Identification and Investigation, OH Grigsby, NL Ohio Bureau of Criminal Identification and Investigation, OH Sallee, PJ Ohio Bureau of Criminal Identification and Investigation, OH (Received 9 August 1996; accepted 24 January 1997) Abstract The sizes of Hae III partial digestion products at D1S7, D2S44, D4S139, D5S110, D10S28, and D17S26 were evaluated in experimentally generated partial digestions of liquid blood DNA. The partial digestion products were highly predictable, suggesting a very high level of sequence conservation in regions flanking variable number tandem repeat (VNTR) blocks. Partial digestion bands associated with three-or-more-banded patterns were also characterized. Partial digestion of three-banded patterns can be used to determine whether the extra bands arise due to internal Hae III sites in the VNTR block and to identify hidden three-banded patterns. Partial digestion products from forensic casework also conformed to size expectations. Presumed partial digestion bands from 27 forensic samples were compared to the experimentally generated data. The causes of partial digestion are examined and recommendations for interpreting forensic DNA evidence exhibiting partial digestion products are given. Keywords: forensic science, band shift, band sizing, DNA typing, gel electrophoresis, restriction fragment length polymorphism, variable number tandem repeat, partial digestion Paper ID: JFS14220J DOI: 10.1520/JFS14220J ASTM International is a member of CrossRef. Author Title Products of Partial Digestion with Hae III. Part 1. Characterization, Casework Experience and Confirmation of the Theory of Three-, Four- and Five-Banded RFLP Pattern Origins Using Partial Digestion Symposium , 0000-00-00 Committee E30