Volume 42, Issue 5 (September 1997)

    Products of Partial Digestion with Hae III. Part 1. Characterization, Casework Experience and Confirmation of the Theory of Three-, Four- and Five-Banded RFLP Pattern Origins Using Partial Digestion

    (Received 9 August 1996; accepted 24 January 1997)

    CODEN: JFSOAD

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    Abstract

    The sizes of Hae III partial digestion products at D1S7, D2S44, D4S139, D5S110, D10S28, and D17S26 were evaluated in experimentally generated partial digestions of liquid blood DNA. The partial digestion products were highly predictable, suggesting a very high level of sequence conservation in regions flanking variable number tandem repeat (VNTR) blocks. Partial digestion bands associated with three-or-more-banded patterns were also characterized. Partial digestion of three-banded patterns can be used to determine whether the extra bands arise due to internal Hae III sites in the VNTR block and to identify hidden three-banded patterns. Partial digestion products from forensic casework also conformed to size expectations. Presumed partial digestion bands from 27 forensic samples were compared to the experimentally generated data. The causes of partial digestion are examined and recommendations for interpreting forensic DNA evidence exhibiting partial digestion products are given.


    Author Information:

    Riech, AK
    Ohio Bureau of Criminal Identification and Investigation, London, OH

    Duewer, DL
    Chemical Sciences and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD

    Benzinger, EA
    Ohio Bureau of Criminal Identification and Investigation, London, OH

    Emerek, EA
    Ohio Bureau of Criminal Identification and Investigation, London, OH

    Deadman, H
    DNA Unit, Room 3905, Federal Bureau of Investigation, Washington, DC

    Thompson, JL
    Ohio Bureau of Criminal Identification and Investigation, London, OH

    Lovekamp, ML
    Ohio Bureau of Criminal Identification and Investigation, London, OH

    Grigsby, NL
    Ohio Bureau of Criminal Identification and Investigation, London, OH

    Sallee, PJ
    Ohio Bureau of Criminal Identification and Investigation, London, OH


    Stock #: JFS14220J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14220J

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    Title Products of Partial Digestion with Hae III. Part 1. Characterization, Casework Experience and Confirmation of the Theory of Three-, Four- and Five-Banded RFLP Pattern Origins Using Partial Digestion
    Symposium , 0000-00-00
    Committee E30