Resident physician, University of Kentucky Chandler Medical Center, Lexington, Kentucky
Associate chief medical examiner, Kentucky Justice Cabinet, Division of Medical Examiner Services, Frankfort, Kentucky
Assistant professor, University of Kentucky Chandler Medical Center, Lexington, Kentucky
Cary Warnecke, S
Forensic scientist specialist, Kentucky State Police Central Forensic Laboratory, Frankfort, Kentucky
(Received 17 June 1996; accepted 11 November 1996)
The examination of azoospermic semen poses a special problem for the forensic scientist. Both serologic and RFLP methods may result in inconclusive results. PCR analysis is known to have an advantage in the evaluation of variably degraded, small quantities of DNA. This investigation addresses the feasibility of detecting the DNA profiles of azoospermic males in cases of suspected rape by the use of PCR amplification of the VNTR locus D1S80. DNA profiles were produced from aspermic semen samples from six vasectomized males. Two mixed postcoital vaginal samples containing azoospermic semen from two of the vasectomized males were also obtained and both revealed the combined profiles of the azoospermic semen donors and the vaginal epithelial donors. All cases resulted in an allelic banding pattern of the donor semen matching the respective blood/saliva standard.
Paper ID: JFS14190J