Journal Published Online: 01 July 1997
Volume 42, Issue 4

DNA Typing of Azoospermic Semen at the D1S80 Locus

CODEN: JFSCAS

Abstract

The examination of azoospermic semen poses a special problem for the forensic scientist. Both serologic and RFLP methods may result in inconclusive results. PCR analysis is known to have an advantage in the evaluation of variably degraded, small quantities of DNA. This investigation addresses the feasibility of detecting the DNA profiles of azoospermic males in cases of suspected rape by the use of PCR amplification of the VNTR locus D1S80. DNA profiles were produced from aspermic semen samples from six vasectomized males. Two mixed postcoital vaginal samples containing azoospermic semen from two of the vasectomized males were also obtained and both revealed the combined profiles of the azoospermic semen donors and the vaginal epithelial donors. All cases resulted in an allelic banding pattern of the donor semen matching the respective blood/saliva standard.

Author Information

Skinker, DM
University of Kentucky Chandler Medical Center, Lexington, Kentucky
Cary Warnecke, S
Kentucky State Police Central Forensic Laboratory, Frankfort, Kentucky
Morrow, JK
University of Kentucky Chandler Medical Center, Lexington, Kentucky
Hunsaker, JC
University of Kentucky Chandler Medical Center, Lexington, Kentucky
Pages: 3
Price: $25.00
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Stock #: JFS14190J
ISSN: 0022-1198
DOI: 10.1520/JFS14190J