ISSN: 0022-1198
CODEN: JFSCA
Page Count: 3

Rapid Screening of Multiple Forensic Stains by SSCP Analysis of HLA-DQα Amplification Products
Lehner, A
Institut für Gerichtliche Medizin, Universität Wien,

Kreike, J
Institut für Gerichtliche Medizin, Universität Wien,

(Received 9 April 1996; accepted 18 November 1996)

Abstract

Single strand conformation polymorphism (SSCP) analysis was performed with HLA-DQα amplification products. The various HLA-DQα genotypes reveal different SSCP patterns. These are reproducible in DNA preparations from unrelated persons with identical HLA-DQα genotype. However, in one of the more frequent HLA-DQα genotypes (1.1/4) two variant SSCP patterns were observed. SSCP analysis allows rapid low-cost screening of many specimens and is an alternative discrimination test of stains beside reverse dot blot hybridization.

Keywords:
forensic science, DNA typing, single strand conformation polymorphism, HLA-DQA1, heteroduplex analysis, polymerase chain reaction

Paper ID: JFS14189J
DOI: 10.1520/JFS14189J
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