Volume 42, Issue 4 (July 1997)

    The Applicability of Formalin-Fixed and Formalin Fixed Paraffin Embedded Tissues in Forensic DNA Analysis

    (Received 26 February 1996; accepted 11 October 1996)

    CODEN: JFSOAD

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    Abstract

    Historically, formalin fixed (FF) tissues could not be used as a source of DNA in forensic science due to the fact that the DNA was too degraded for DNA analysis. With the introduction of the polymerase chain reaction (PCR) technique to forensic science, the usefulness of DNA from this biological material has been re-evaluated. This study evaluates the potential use of DNA from FF and formalin fixed paraffin embedded (FFPE) tissues in 13 PCR systems; HLA DQα, LDLR, GYPA, HBGG, D7S8, GC, D1S80, vWA31, THO1, F13A1, FES/FPS, TPOX, and CSF1PO. The first six, HLA DQα, LDLR, GYPA, HBGG, D7S8, and GC are reverse dot blot systems, D1S80 is an amplified fragment length polymorphism (AmpFlp) system and the others are short tandem repeats (STRs). This study shows that FFPE tissue which has not been fixed in formalin for more than three days is a useful source of DNA for 12 of the 13 PCR systems. In contrast, FF tissue did not prove to be a reliable source of DNA for the PCR techniques examined here.


    Author Information:

    Juston, AC
    Forensic scientist trainee and supervisor, Suffolk County Crime Laboratory, North County Complex, Hauppauge, NY

    Henry, BE
    Technical Coordinator, Alabama Department of Forensic Science, Birmingham, AL

    Ballantyne, J
    Forensic scientist trainee and supervisor, Suffolk County Crime Laboratory, North County Complex, Hauppauge, NY

    Romero, RL
    Criminalist, Washoe County Sheriff's Office, Forensic Science Division, Reno, NV


    Stock #: JFS14188J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14188J

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    Author
    Title The Applicability of Formalin-Fixed and Formalin Fixed Paraffin Embedded Tissues in Forensic DNA Analysis
    Symposium , 0000-00-00
    Committee E30