Volume 42, Issue 4 (July 1997)

    Inhibition of Ethanol Production by Saccharomyces cerevisiae in Human Blood by Sodium Fluoride

    (Received 15 July 1996; accepted 11 November 1996)

    CODEN: JFSOAD

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    Abstract

    Production of ethanol in antemortem blood samples inoculated with an efficient ethanol-producing microorganism and incubated at various temperatures is discussed. Whole blood samples inoculated with Saccharomyces cerevisiae were incubated in gray stoppered Venoject® tubes (approximate draw volume 7 mL) containing sodium fluoride (17.5 mg) and potassium oxalate (14.0 mg) at 4°C, 25°C, and 37°C for 0, 24, 96, 192, and 408 h. No volatile substances (such as ethanol, methanol, isopropanol, acetone, or acetaldehyde) (<0.010 g/dL) were produced in any of the samples at 4 or 25°C. At 24 h incubation a trace amount (<0.018 g/dL) of ethanol was detected at 37°C.


    Author Information:

    Amick, GD
    Forensic toxicologist and chief toxicologist, South Carolina Law Enforcement Division, Columbia, SC

    Habben, KH
    Forensic toxicologist and chief toxicologist, South Carolina Law Enforcement Division, Columbia, SC


    Stock #: JFS14184J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14184J

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    Title Inhibition of Ethanol Production by Saccharomyces cerevisiae in Human Blood by Sodium Fluoride
    Symposium , 0000-00-00
    Committee E30