Volume 42, Issue 4 (July 1997)
Inhibition of Ethanol Production by Saccharomyces cerevisiae in Human Blood by Sodium Fluoride
Production of ethanol in antemortem blood samples inoculated with an efficient ethanol-producing microorganism and incubated at various temperatures is discussed. Whole blood samples inoculated with Saccharomyces cerevisiae were incubated in gray stoppered Venoject® tubes (approximate draw volume 7 mL) containing sodium fluoride (17.5 mg) and potassium oxalate (14.0 mg) at 4°C, 25°C, and 37°C for 0, 24, 96, 192, and 408 h. No volatile substances (such as ethanol, methanol, isopropanol, acetone, or acetaldehyde) (<0.010 g/dL) were produced in any of the samples at 4 or 25°C. At 24 h incubation a trace amount (<0.018 g/dL) of ethanol was detected at 37°C.