ISSN: 0022-1198
CODEN: JFSCA
Page Count: 4

PCR-Based Forensic Testing of DNA from Stained Cytological Smears
Brandt-Casadevall, C
Institut de Médecine Légale,

Grange, F
Institut de Médecine Légale,

Dimo-Simonin, N
Institut de Médecine Légale,

(Received 4 March 1996; accepted 30 September 1996)

Abstract

Experiments were performed to evaluate the efficiency of PCR-STR (Short Tandem Repeats) and PCR-sequence polymorphisms for the identification of stained pap smears and postocital slides stained with cytological and forensic techniques. HLA-DQA1, PolyMarker, Amelogenin, HUMTH01, HUMVWFA31, HUMF13B, and HUMFES/FPS were determined. With the exception of the forensic Baecchi stain, all the PCR-systems gave consistent results in comparison with the reference blood from the donors. Cytological stained smears can be important evidence for identification in sexual assault cases and in missing person cases.

Keywords:
forensic science, DNA typing, polymerase chain reaction, short tandem repeat, sexual assault evidence, HLA-DQA1, LDLR, HBGG, GYPA, D758, GC, TH01, VWFA31, F13B, FES/FPS, amelogenin, Pap smears, cytology slides, histological stains

Paper ID: JFS14157J
DOI: 10.1520/JFS14157J
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