Volume 42, Issue 3 (May 1997)

    Defining Microsatellite Alleles by Genotyping Global Indigenous Human Populations and Non-Human Primates

    (Received 27 July 1995; accepted 23 September 1996)

    CODEN: JFSOAD

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    Abstract

    Polymorphisms at variable number of tandem repeat (VNTR) loci have been used in forensic science for almost decade. Microsatellite loci, especially tri-, tetra-, and pentanucleotide repeat loci, have shown great potential in personal identification and paternity testing. In this report, we describe results of genotyping three tetranucleotide repeat loci (D5S818, D7S820, D13S317) in 16 worldwide indigenous human populations and one chimpanzee population which were being developed for forensic applications. We demonstrate the utility of typing globally diverse populations in defining microsatellite alleles: Specifically (i) investigating the measurement errors of each allele using semi-automatic genotyping instrumentation and software, (ii) assessing the range of alleles, (iii) understanding the extent of allele frequency differences across worldwide populations, and (iv) identifying possible anomalous alleles with complex structures.


    Author Information:

    Robertson, JM
    Applied Biosystem Division, Perkin Elmer, Inc., Foster City, CA

    Jin, L
    Stanford University School of Medicine, Stanford, CA

    Buoncristiani, M
    Applied Biosystem Division, Perkin Elmer, Inc., Foster City, CA

    Underhill, PA
    Applied Biosystem Division, Perkin Elmer, Inc., Foster City, CA


    Stock #: JFS14154J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14154J

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    Author
    Title Defining Microsatellite Alleles by Genotyping Global Indigenous Human Populations and Non-Human Primates
    Symposium , 0000-00-00
    Committee E30