Summary of Validation Studies from Twenty Six Forensic Laboratories in the United States and Canada on the Use of the AmpliType® PM PCR Amplification and Typing Kit

Volume 42, Issue 1 (January 1997)

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ISSN: 0022-1198
CODEN: JFSCA
Page Count: 10

Summary of Validation Studies from Twenty-Six Forensic Laboratories in the United States and Canada on the Use of the AmpliType® PM PCR Amplification and Typing Kit
Word, CJ
Cellmark Diagnostics, MD

Bing, DH
Director of Clinical Testing, Clinical Testing Laboratory, CBR Laboratories/The Center for Blood Research, MA

Sawosik, TM
Medical writer, Parexel, MA

(Received 28 September 1995; accepted 29 May 1996)

Abstract

A cooperative study was undertaken to collect and summarize the results of validation studies from forensic laboratories in the United States and Canada on the use of the AmpliType® PM PCR amplification and typing kit for genetic typing of forensic biological evidence. This report compiles data from 26 laboratories on: 1) reproducibility studies on DNA extracted from various samples, 2) genetic typing of DNA extracted from a variety of biological samples on various substrates, 3) the effects of exogenous chemicals, materials, and environmental factors on test results, 4) sensitivity studies to determine the least detectable amount of extracted genomic DNA that can be reliably typed, 5) analysis of mixtures containing two sources of genomic DNA, 6) cross-hybridization with DNA extracted from various nonhuman species, and 7) evaluation of assay performance on parallel studies with other genetic typing systems on proficiency test panels, mock cases, and adjudicated/nonprobative casework. Equivalent results were obtained by each laboratory that supplied data, demonstrating the reliability and consistency of the test. Overall, it can be concluded from this study that the AmpliType PM PCR amplification and typing kit meets the guidelines of the Technical Working Group on DNA Analysis Methods (TWGDAM) and there is general scientific acceptability of this kit for forensic DNA testing.

Keywords:
forensic science, polymerase chain reaction, DNA typing, PM, polymarker, LDLR, GYPA, HBGG, D7S8, GC

Paper ID: JFS14066J
DOI: 10.1520/JFS14066J
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Author Title Summary of Validation Studies from Twenty-Six Forensic Laboratories in the United States and Canada on the Use of the AmpliType® PM PCR Amplification and Typing Kit Symposium , 0000-00-00 Committee E30

		SEDL / Journals / Journal of Forensic Sciences (JOFS) / Citation Page Volume 42, Issue 1 (January 1997) Click here to download this paper now for $25 PDF (512K) View License Agreement ISSN: 0022-1198 CODEN: JFSCA Page Count: 10 Summary of Validation Studies from Twenty-Six Forensic Laboratories in the United States and Canada on the Use of the AmpliType® PM PCR Amplification and Typing Kit Word, CJ Cellmark Diagnostics, MD Bing, DH Director of Clinical Testing, Clinical Testing Laboratory, CBR Laboratories/The Center for Blood Research, MA Sawosik, TM Medical writer, Parexel, MA (Received 28 September 1995; accepted 29 May 1996) Abstract A cooperative study was undertaken to collect and summarize the results of validation studies from forensic laboratories in the United States and Canada on the use of the AmpliType® PM PCR amplification and typing kit for genetic typing of forensic biological evidence. This report compiles data from 26 laboratories on: 1) reproducibility studies on DNA extracted from various samples, 2) genetic typing of DNA extracted from a variety of biological samples on various substrates, 3) the effects of exogenous chemicals, materials, and environmental factors on test results, 4) sensitivity studies to determine the least detectable amount of extracted genomic DNA that can be reliably typed, 5) analysis of mixtures containing two sources of genomic DNA, 6) cross-hybridization with DNA extracted from various nonhuman species, and 7) evaluation of assay performance on parallel studies with other genetic typing systems on proficiency test panels, mock cases, and adjudicated/nonprobative casework. Equivalent results were obtained by each laboratory that supplied data, demonstrating the reliability and consistency of the test. Overall, it can be concluded from this study that the AmpliType PM PCR amplification and typing kit meets the guidelines of the Technical Working Group on DNA Analysis Methods (TWGDAM) and there is general scientific acceptability of this kit for forensic DNA testing. Keywords: forensic science, polymerase chain reaction, DNA typing, PM, polymarker, LDLR, GYPA, HBGG, D7S8, GC Paper ID: JFS14066J DOI: 10.1520/JFS14066J ASTM International is a member of CrossRef. Author Title Summary of Validation Studies from Twenty-Six Forensic Laboratories in the United States and Canada on the Use of the AmpliType® PM PCR Amplification and Typing Kit Symposium , 0000-00-00 Committee E30