Volume 41, Issue 6 (November 1996)

    PCR Analysis of Lewis-Negative Gene Mutations and the Distribution of Lewis Alleles in a Japanese Population

    (Received 28 September 1995; accepted 18 March 1996)

    CODEN: JFSOAD

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    Abstract

    Three mutations in the Lewis-negative gene, T59G, G508A and T1067A, have been detected by means of a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 149 unrelated Japanese individuals. We found three common Lewis alleles—Le (without the T59G, G508A, and T1067A mutations), le1 (with the T59G and G508A mutations), and le2 (with the T59G and T1067A mutations) in a Japanese population. In addition, we also found one rare Lewis-negative allele, le3 (only with the T1067A mutation). The allele frequencies of Le, le1, le2, and le3 were 0.607, 0.275, 0.114, and 0.003, respectively. Our results were in accordance with those expected by the Hardy-Weinberg equilibrium. Some statistical parameters of forensic interest were also calculated.


    Author Information:

    Kimura, H
    Visiting assistant professor, assistant professor, lecturer, postgraduate student, and professor, Kurume University School of Medicine, Kurume, Fukuoka

    Soejima, M
    Visiting assistant professor, assistant professor, lecturer, postgraduate student, and professor, Kurume University School of Medicine, Kurume, Fukuoka

    Uchida, N
    Visiting assistant professor, assistant professor, lecturer, postgraduate student, and professor, Kurume University School of Medicine, Kurume, Fukuoka

    Koda, Y
    Visiting assistant professor, assistant professor, lecturer, postgraduate student, and professor, Kurume University School of Medicine, Kurume, Fukuoka

    Liu, Y
    Visiting assistant professor, assistant professor, lecturer, postgraduate student, and professor, Kurume University School of Medicine, Kurume, Fukuoka


    Stock #: JFS14040J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14040J

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    Title PCR Analysis of Lewis-Negative Gene Mutations and the Distribution of Lewis Alleles in a Japanese Population
    Symposium , 0000-00-00
    Committee E30