ISSN: 0022-1198
CODEN: JFSCA
Page Count: 6

Use of REMEDi HS in Emergency Toxicology for a Rapid Estimate of Drug Concentrations in Urine, Serum, and Gastric Samples
Lai, JS
Clinical Systems Division, Bio-Rad Laboratories, CA

Ohtsuji, M
Kanazawa University, Faculty of Medicine,

Binder, SR
Clinical Systems Division, Bio-Rad Laboratories, CA

Kondo, T
Kanazawa University, Faculty of Medicine,

Ohshima, T
Kanazawa University, Faculty of Medicine,

Takayasu, T
Kanazawa University, Faculty of Medicine,

(Received 11 August 1995; accepted 8 February 1996)

Abstract

The REMEDi HS is a broad spectrum drug identification system, designed for emergency toxicology screening and forensic applications. The total analysis time is about 20 min. The current library has 555 drugs and metabolites. The system has a software routine that uses an internal standard (IS) to perform quantitative analysis for target compounds when calibrators are available; further, response factors (RF) are supplied for a rapid estimate of drug concentrations when calibrators are unavailable. In the present study, the concentrations of six drugs (bromisovalum, ephedrine, hydroxyzine, diphenhydramine, ranitidine, and lidocaine) and a metabolite of lidocaine (glycinexylidide) were determined using both methods. The slopes of the regression lines between the rapid estimate method and the IS method were generally within 20% of unity, in agreement with the manufacturer's claim. Semiquantitative estimates based on RF also showed good agreement with results obtained using multipoint calibration. These estimates were sufficient for clinical differentiation of routine and toxic levels. Our study demonstrated that the REMEDi HS is particularly useful for a rapid estimate of drug concentrations in the samples from emergency cases when calibrators are not readily available. Our study also showed that this system can be used for the therapeutic monitoring of ranitidine, bromisovalum, lidocaine, and diphenhydramine.

Keywords:
forensic science, forensic toxicology, REMEDi HS, emergency medicine, bromisovalum, ephedrine, hydroxyzine, diphenhydramine, ranitidine, lidocaine, glycinexylidide

Paper ID: JFS14017J
DOI: 10.1520/JFS14017J
ASTM International is a member of CrossRef.