Volume 41, Issue 5 (September 1996)

    A New Individualization Marker of Sweat: Deoxyribonuclease I (DNase I) Polymorphism

    (Received 17 October 1995; accepted 22 January 1996)

    CODEN: JFSOAD

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    Abstract

    We have confirmed for the first time, both biochemically and immunologically, the existence of deoxyribonuclease I (DNase I) in human liquid sweat. Isoelectric focusing of sweat samples on polyacrylamide gels (pH 3.5 to 5), followed by dried agarose film overlay detection, was used to determine the phenotypes of sweat DNase I. Because this detection method not only had high sensitivity, but also high band resolution, it was possible to determine DNase I types from sweat samples of 50 to 100 μL. Pretreatment of sweat samples with sialidase was essential for typing to enhance markedly the sensitivity accompanied by simplification of the isozyme pattern. The DNase I types in all sweat samples were consistently related to the types found in corresponding blood, urine, and semen samples. DNase I typing could, therefore, provide a novel discriminant characteristic in the forensic examination of sweat.


    Author Information:

    Nadano, D
    Forensic biologists, Fukui Medical School, Maebashi, Gunma,

    Takeshita, H
    Forensic biologists, Fukui Medical School, Maebashi, Gunma,

    Kishi, K

    Sawazaki, K

    Iida, R
    Forensic biologists, Fukui Medical School, Maebashi, Gunma,

    Miyahara, S
    Forensic biologists, Fukui Medical School, Maebashi, Gunma,

    Yasuda, T


    Stock #: JFS14012J

    ISSN: 0022-1198

    DOI: 10.1520/JFS14012J

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    Author
    Title A New Individualization Marker of Sweat: Deoxyribonuclease I (DNase I) Polymorphism
    Symposium , 0000-00-00
    Committee E30