Purification and Determination Procedure of Coumarin Derivatives

    Volume 41, Issue 4 (July 1996)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 4


    Seo, BS
    Catholic University Medical College, Seoul,

    Kim, DH
    Division of Chemical Analysis, The National Institute of Scientific Investigation, Seoul,

    Kim, EH
    Division of Chemical Analysis, The National Institute of Scientific Investigation, Seoul,

    Paeng, K-J
    Yonsei University, Wonju,

    Park, SW
    Division of Chemical Analysis, The National Institute of Scientific Investigation, Seoul,

    (Received 26 June 1995; accepted 13 November 1995)

    Abstract

    Anticoagulant coumarin derivatives, bromadiolone, brodifacoum, warfarin, and coumatetralyl were analyzed simultaneously with a modified analytical method. The method includes purification by Sep-Pak cartridge and HPLC analysis using isocratic elution with methanol: 0.8% Acetic acid (8:2). The recoveries of coumarin derivatives from biological samples ranged from 96 to 99%. The concentrations of coumarin derivatives in intoxicated rats' organ were also determined with this analytical procedure.


    Paper ID: JFS13979J

    DOI: 10.1520/JFS13979J

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    Author
    Title Purification and Determination Procedure of Coumarin Derivatives
    Symposium , 0000-00-00
    Committee E30