Multiplex Amplification and Typing Procedure for the Loci D1S80 and Amelogenin

    Volume 41, Issue 4 (July 1996)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 4


    Koons, BW
    Chemists, Forensic Science Research Unit, Forensic Science Research and Training Center, FBI Laboratory, FBI Academy, Quantico,

    Budowle, B
    Chief, Forensic Science Research Unit, Forensic Science Research and Training Center, FBI Laboratory, FBI Academy, Quantico,

    Errera, JD
    Chemists, Forensic Science Research Unit, Forensic Science Research and Training Center, FBI Laboratory, FBI Academy, Quantico,

    (Received 13 July 1995; accepted 1 November 1995)

    Abstract

    A method has been developed that enables multiplex amplification and simultaneous typing of the loci D1S80 and amelogenin using discontinuous polyacrylamide gel electrophoresis and silver staining. The protocol is sensitive, simple, rapid, and relatively inexpensive. The results of the multiplex analysis of the D1S80 and amelogenin loci were comparable to those obtained when each locus was analyzed individually. A small validation study was undertaken to evaluate the forensic applicability of this multiplex system. The data demonstrate that DNA exposed to a variety of environmental insults yields reliable multiplex typing results.


    Paper ID: JFS13973J

    DOI: 10.1520/JFS13973J

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    Author
    Title Multiplex Amplification and Typing Procedure for the Loci D1S80 and Amelogenin
    Symposium , 0000-00-00
    Committee E30