Volume 41, Issue 4 (July 1996)
Allelic Ladder Characterization of the Short Tandem Repeat Polymorphism in Intron 6 of the Lipoprotein Lipase Gene and Its Application in an Austrian Caucasian Population Study
The short tandem repeat (STR) polymorphism HumLPL (TTTA)n, which is located in intron 6 of the lipoprotein lipase gene, was investigated by AMPFLP (amplification fragment length polymorphism)-technique using an allelic ladder consisting of amplified alleles of this locus as a standard size marker.
The allelic ladder was prepared by pooling equal concentrations of six separate alleles, which were identified by their different electrophoretic mobility in native polyacrylamide gel, eluted and subsequently amplified. Sequence analysis of the ladder alleles and allele 7, which is not included in the ladder, showed a regular repeat structure with 7 and 9 to 14 repetitions of the core repeat.
The allelic ladder was employed in the analysis of the genotypes of 550 unrelated Caucasoids of Austria. No new alleles were found.
The population investigated showed no deviation from Hardy-Weinberg equilibrium (P = 0.195).