Volume 41, Issue 3 (May 1996)

    PCR-Based Human Leukocyte Antigen (HLA) DQα Typing of Blood Stained Light and Dark Blue Denim Fabric

    (Received 14 August 1995; accepted 19 October 1995)

    CODEN: JFSOAD

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    Abstract

    Obtaining typable PCR products from DNA purified from blood stained blue denim has been difficult since inhibitors of PCR in blue denim apparently co-purify with the DNA. Organic and chelex extraction methods were tested for their ability to purify typable DNA from either light or dark blue denim fabric both stained with blood. DNA purified from the light blue denim using either method was successfully used in obtaining correct HLA-DQα typing results. The chelex, but not the organic, procedure was able to yield typable DNA when the dark blue denim was the substrate. Therefore, the chelex method may be more effective than the organic method in preventing compounds that inhibit PCR from co-purifying with the DNA.


    Author Information:

    Marino, MA
    Research Associate, Deputy Branch Chief, and Acting Branch Chief, Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, Rockville, MD

    Belgrader, P
    Research Associate, Deputy Branch Chief, and Acting Branch Chief, Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, Rockville, MD

    Del Rio, SA
    Research Associate, Deputy Branch Chief, and Acting Branch Chief, Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, Rockville, MD


    Stock #: JFS13941J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13941J

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    Author
    Title PCR-Based Human Leukocyte Antigen (HLA) DQα Typing of Blood Stained Light and Dark Blue Denim Fabric
    Symposium , 0000-00-00
    Committee E30