You are being redirected because this document is part of your ASTM Compass® subscription.
    This document is part of your ASTM Compass® subscription.

    Volume 41, Issue 3 (May 1996)

    Stability and Spontaneous Production of Blood Cyanide During Heating

    (Received 2 August 1995; accepted 29 August 1995)

    CODEN: JFSOAD

      Format Pages Price  
    PDF 4 $25   ADD TO CART

    Cite this document

    X Add email address send
    X
      .RIS For RefWorks, EndNote, ProCite, Reference Manager, Zoteo, and many others.   .DOCX For Microsoft Word



    Abstract

    To investigate the effects of heat on blood cyanide concentrations, in vitro experiments were performed using a headspace gas chromatographic method. Cyanide concentrations were determined for solutions of human hemoglobin (Hb) at neutrality, and for blood which was sealed in a vial and incubated at 25, 50, 63, 75 and 90°C for 1 h. Spontaneous cyanide production was also measured. Nearly all of the added cyanide was recovered in both the Hb and for blood samples which were heated below 63°C. Cyanide recovery in Hb decreased in a temperature-dependent manner at temperatures above 75°C, and more than half of the recovered cyanide was found to be in the free form. In contrast, cyanide in blood disappeared more rapidly, and a major portion of it existed in the bound form. Cyanide concentrations in Hb solutions which were heated at 90°C dropped in the two phases; a rapid initial phase, followed by a slower process. Spontaneous cyanide production was observed at temperatures above 50°C for Hb and above 63°C for blood. Under optimal conditions (75°C heating), about 0.2 mmol of cyanide was produced per mol heme of Hb.


    Author Information:

    Seto, Y
    Senior Research Official, National Research Institute of Police Science, Tokyo,


    Stock #: JFS13936J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13936J

    ASTM International
    is a member of CrossRef.

    Author
    Title Stability and Spontaneous Production of Blood Cyanide During Heating
    Symposium , 0000-00-00
    Committee E30