ISSN: 0022-1198
CODEN: JFSCA
Page Count: 4

HUMTH01 Validation Studies: Effect of Substrate, Environment, and Mixtures
Andrew, IR
Forensic Officer, Premier Caseworker, and Forensic Officers, Victoria Forensic Science Centre (VFSC) Forensic Drive, Victoria

Hedley, JA
Forensic Officer, Premier Caseworker, and Forensic Officers, Victoria Forensic Science Centre (VFSC) Forensic Drive, Victoria

van Oorschot, RAH
Forensic Officer, Premier Caseworker, and Forensic Officers, Victoria Forensic Science Centre (VFSC) Forensic Drive, Victoria

Gutowski, SJ
Forensic Officer, Premier Caseworker, and Forensic Officers, Victoria Forensic Science Centre (VFSC) Forensic Drive, Victoria

Robinson, SL
Forensic Officer, Premier Caseworker, and Forensic Officers, Victoria Forensic Science Centre (VFSC) Forensic Drive, Victoria

(Received 17 February 1995; accepted 23 June 1995)

Abstract

New DNA typing methods need to be thoroughly validated prior to use in forensic investigations. This includes determining the effects different sample conditions have on the typeability of those samples. Biological samples routinely encountered in forensic case work were exposed to a series of different substrates, environmental conditions, and mixtures and typed for the STR HUMTH01 using PCR. None of the conditions resulted in a false typing or preferential allele amplification. It is demonstrated that the application of HUMTH01 typing methods in forensic case work can be reliable, robust, and efficient.

Keywords:
forensic science, HUMTH01, DNA, PCR, validation, substrates, environments, mixtures

Paper ID: JFS13912J
DOI: 10.1520/JFS13912J
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