Volume 41, Issue 1 (January 1996)

    A PCR-Based Strategy for ABO Genotype Determination

    (Received 23 March 1995; accepted 5 June 1995)

    CODEN: JFSOAD

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    Abstract

    The ABO blood group system has been widely used in forensic serology. Several techniques have been developed which detect ABH antigens. To overcome the problems associated with conventional methods such as bacterial contamination, extreme environmental conditions, antigen activity, non-secretor issues, and non-specific absorption, several new strategies have been employed to detect ABO genotypes by PCR. We have developed improved amplimers for the glycosyl transferase locus on chromosome 9 and examined the suitability of PCR-based ABO genotyping for forensic identification. We show that the ABO system is primate specific and that DNA extracted from various tissues commonly encountered in criminal cases can be quickly and reliably typed by ABO-PCR. The results indicate that ABO genotyping by PCR and restriction enzyme digestion of the amplified product is a useful procedure for forensic analysis that can provide additional discriminating power compared to conventional immunological methods.


    Author Information:

    Scherczinger, CA
    Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Ladd, C
    Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Pagliaro, EM
    Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Lee, HC
    Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Bourke, MT
    Connecticut State Police Forensic Science Laboratory, Meriden, CT

    Gaensslen, RE
    Professor and Director, Forensic Sciences, University of New Haven, West Haven, CT


    Stock #: JFS13910J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13910J

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    Author
    Title A PCR-Based Strategy for ABO Genotype Determination
    Symposium , 0000-00-00
    Committee E30