Volume 41, Issue 1 (January 1996)

    Stability of Ecgonine Methyl Ester in Postmortem Urine Specimens

    (Received 3 May 1995; accepted 16 May 1995)

    CODEN: JFSOAD

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    Abstract

    In this study, 25 postmortem urine specimens testing positive, for cocaine and ecogonine methyl ester (EME) by full scan electron impact gas chromatography/mass spectrometry, were used to evaluate the stability of EME in refrigerated and frozen conditions. After an initial quantitation (t = 0), these specimens were split and stored at either 4°C or −20°C. At several intervals, over a six month period, the specimens were tested for cocaine and EME. Twenty-two of the frozen specimens were within 20% of their t = 0 EME concentration after 6 months; 19 of the 25 refrigerated specimens showed similar stability. At least 50% of the EME present was detected in all specimens under both storage conditions. In addition, there was no evidence to suggest that EME concentrations increased over time even though decreases in cocaine concentrations were observed over the same time period. This suggests that the presence of EME in urine specimens indicates in vivo conversion of cocaine and, therefore, use of cocaine.


    Author Information:

    Levine, B
    Office of the Chief Medical Examiner, State of Maryland, Baltimore, MD

    Smialek, JE
    Office of the Chief Medical Examiner, State of Maryland, Baltimore, MD

    Ramcharitar, V
    Office of the Chief Medical Examiner, State of Maryland, Baltimore, MD


    Stock #: JFS13908J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13908J

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    Author
    Title Stability of Ecgonine Methyl Ester in Postmortem Urine Specimens
    Symposium , 0000-00-00
    Committee E30