Volume 40, Issue 4 (July 1995)

    Confirmation of the Identity of Human Skeletal Remains Using Multiplex PCR Amplification and Typing Kits

    (Received 12 October 1994; accepted 8 December 1994)

    CODEN: JFSOAD

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    Abstract

    The identity of human skeletal remains found in a wooded area approximately one year after the person was reported missing was provisionally established by routine methods and circumstantial evidence. Multiplex PCR systems—the AmpliType® PM PCR Amplification and Typing Kit and the GenePrint™ STR Triplex Amplification and Typing Kit—were used to confirm the identification. DNA profiles from femur bone from the remains were compared with profiles derived from head hairs from a hairbrush recovered in the missing woman's apartment. In addition, a sex typing procedure using the X-Y homologous gene amelogenin was carried out. This is the first report of a case using commercially available multiplex PCR amplification and typing kits to confirm the identity of skeletal remains.


    Author Information:

    Budowle, B
    Research Chemist, Forensic Science Research and Training Center, Laboratory Division, Federal Bureau of Investigation Academy, Quantico, VA

    Hochmeister, MN
    Research Scientists, and Director, DNA Identity Laboratory, Institute of Legal Medicine, University of Berne, Berne,

    Bohnert, M
    Forensic Pathologist, Institute of Legal Medicine, University of Freiburg im Breisgau,

    Rudin, O
    Research Scientists, and Director, DNA Identity Laboratory, Institute of Legal Medicine, University of Berne, Berne,

    Dirnhofer, R
    Research Scientists, and Director, DNA Identity Laboratory, Institute of Legal Medicine, University of Berne, Berne,

    Borer, UV
    Research Scientists, and Director, DNA Identity Laboratory, Institute of Legal Medicine, University of Berne, Berne,


    Stock #: JFS13855J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13855J

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    Title Confirmation of the Identity of Human Skeletal Remains Using Multiplex PCR Amplification and Typing Kits
    Symposium , 0000-00-00
    Committee E30