ISSN: 0022-1198
CODEN: JFSCA
Page Count: 5

Confirmation of the Identity of Human Skeletal Remains Using Multiplex PCR Amplification and Typing Kits
Budowle, B
Research Chemist, Forensic Science Research and Training Center, Laboratory Division, Federal Bureau of Investigation Academy, VA

Hochmeister, MN
Research Scientists, and Director, DNA Identity Laboratory, Institute of Legal Medicine, University of Berne,

Bohnert, M
Forensic Pathologist, Institute of Legal Medicine, University of Freiburg im Breisgau,

Rudin, O
Research Scientists, and Director, DNA Identity Laboratory, Institute of Legal Medicine, University of Berne,

Dirnhofer, R
Research Scientists, and Director, DNA Identity Laboratory, Institute of Legal Medicine, University of Berne,

Borer, UV
Research Scientists, and Director, DNA Identity Laboratory, Institute of Legal Medicine, University of Berne,

(Received 12 October 1994; accepted 8 December 1994)

Abstract

The identity of human skeletal remains found in a wooded area approximately one year after the person was reported missing was provisionally established by routine methods and circumstantial evidence. Multiplex PCR systems—the AmpliType® PM PCR Amplification and Typing Kit and the GenePrint™ STR Triplex Amplification and Typing Kit—were used to confirm the identification. DNA profiles from femur bone from the remains were compared with profiles derived from head hairs from a hairbrush recovered in the missing woman's apartment. In addition, a sex typing procedure using the X-Y homologous gene amelogenin was carried out. This is the first report of a case using commercially available multiplex PCR amplification and typing kits to confirm the identity of skeletal remains.

Keywords:
forensic science, human identification, deoxyribonucleic acid (DNA), polymerase chain reaction (PCR), genetic typing, HLA DQA1, LDLR, GYPA, HBGG, D7S8, GC, short tandem repeats (STR), TH01, TPOX, CSF1PO, amelogenin, sexing

Paper ID: JFS13855J
DOI: 10.1520/JFS13855J
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