Typing of DNA HLA-DQα Alleles Extracted from Human Nail Material Using Polymerase Chain Reaction

    Volume 40, Issue 4 (July 1995)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 3


    Tahir, MA
    Chief of Serology and DNA Unit, Indianapolis-Marion County Forensic Services Agency, Indianapolis, IN

    Watson, N
    Forensic Science Unit, University of Strathclyde, Glasgow,

    (Received 10 June 1994; accepted 26 October 1994)

    Abstract

    The deoxyribonucleic acid (DNA) typing of human Leukocyte Antigen (HLA) DQα from human fingernails is described. HLA-DQα genotypes can be accurately determined from clipped fingernails. We have typed 26 nails accurately, while one did not give any type since that one sample did not amplify due to the low quantity of DNA. The cut off limit for the digested material to be amplified is approximately 9 mgs of nail material.


    Paper ID: JFS13839J

    DOI: 10.1520/JFS13839J

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    Title Typing of DNA HLA-DQα Alleles Extracted from Human Nail Material Using Polymerase Chain Reaction
    Symposium , 0000-00-00
    Committee E30