Volume 40, Issue 4 (July 1995)
Typing of DNA HLA-DQα Alleles Extracted from Human Nail Material Using Polymerase Chain Reaction
The deoxyribonucleic acid (DNA) typing of human Leukocyte Antigen (HLA) DQα from human fingernails is described. HLA-DQα genotypes can be accurately determined from clipped fingernails. We have typed 26 nails accurately, while one did not give any type since that one sample did not amplify due to the low quantity of DNA. The cut off limit for the digested material to be amplified is approximately 9 mgs of nail material.