ISSN: 0022-1198
CODEN: JFSCA
Page Count: 3

Typing of DNA HLA-DQα Alleles Extracted from Human Nail Material Using Polymerase Chain Reaction
Tahir, MA
Chief of Serology and DNA Unit, Indianapolis-Marion County Forensic Services Agency, IN

Watson, N
Forensic Science Unit, University of Strathclyde,

(Received 10 June 1994; accepted 26 October 1994)

Abstract

The deoxyribonucleic acid (DNA) typing of human Leukocyte Antigen (HLA) DQα from human fingernails is described. HLA-DQα genotypes can be accurately determined from clipped fingernails. We have typed 26 nails accurately, while one did not give any type since that one sample did not amplify due to the low quantity of DNA. The cut off limit for the digested material to be amplified is approximately 9 mgs of nail material.

Keywords:
pathology and biology, deoxyribonucleic acid, DNA polymorphism, genetic typing, HLA-DQα, locus, polymerase chain reaction, fingernails

Paper ID: JFS13839J
DOI: 10.1520/JFS13839J
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