Testing Human Hair for Cannabis II. Identification of THC-COOH by GC-MS-NCI as a Unique Proof

    Volume 40, Issue 4 (July 1995)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 4


    Kintz, P
    Institut de Medecine Legale, Strasbourg,

    Mangin, P
    Institut de Medecine Legale, Strasbourg,

    Cirimele, V
    Institut de Medecine Legale, Strasbourg,

    (Received 18 October 1994; accepted 15 December 1994)

    Abstract

    To validate information on cannabis use, 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic (THC-COOH) was investigated in human hair. The identification of THC-COOH in hair would document cannabis use more effectively than the detection of the parent drug which might have come from environmental exposure in a smoky atmosphere. Samples (100 mg) were decontaminated with methylene chloride and destroyed by incubation in 1 mL of 1 N sodium hydroxide for 30 min at 95°C in presence of 10 ng of THC-COOH-d3. After cooling, samples were extracted by n-hexane/ethyl acetate after acidification with acetic acid. After derivatization by PFPA-PFP-OH of the dry extract, drugs were separated on a HP1 capillary column, and detected by mass spectrometry (m/z 602) using negative chemical ionization with methane as reagent gas. Among 30 samples obtained from subjects deceased from fatal heroin overdose, 17 tested positive for THC-COOH, in the range 0.02–0.39 ng/mg, with an average of 0.12 ng/mg.


    Paper ID: JFS13837J

    DOI: 10.1520/JFS13837J

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    Title Testing Human Hair for Cannabis II. Identification of THC-COOH by GC-MS-NCI as a Unique Proof
    Symposium , 0000-00-00
    Committee E30