Volume 40, Issue 3 (May 1995)

    Sensitive Detection of Strychnine in Biological Samples by Gas Chromatography with Surface Ionization Detection

    (Received 24 March 1994; accepted 11 October 1994)

    CODEN: JFSOAD

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    Abstract

    Strychnine was found measurable with high sensitivity by gas chromatography (GC) with surface ionization detection (SID) and using brucine as the internal standard. The calibration curve was linear in the range of 62.5 to 1000 pg, on column. The detection limit of strychnine was about 50 pg on column (2.5 ng/mL sample); the sensitivity was 70 times higher than that reported by high-performance liquid chromatography. A detailed procedure for extraction of strychnine from human whole blood and urine was established using Sep-Pak C18 cartridges; the recovery of strychnine and brucine, which had been spiked in both body fluids, was about 90%.


    Author Information:

    Ishii, A
    Research Associate, Assistant Professor, and Professor, Hamamatsu University School of Medicine, Hamamatsu,

    Hattori, H
    Associate Professor, Aichi Medical University, Aichi,

    Seno, H
    Research Associate, Assistant Professor, and Professor, Hamamatsu University School of Medicine, Hamamatsu,

    Suzuki, O
    Research Associate, Assistant Professor, and Professor, Hamamatsu University School of Medicine, Hamamatsu,

    Kumazawa, T
    Assistant Professor, Showa University School of Medicine, Tokyo,


    Stock #: JFS13810J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13810J

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    Title Sensitive Detection of Strychnine in Biological Samples by Gas Chromatography with Surface Ionization Detection
    Symposium , 0000-00-00
    Committee E30