ISSN: 0022-1198
CODEN: JFSCA
Page Count: 5

Restriction Fragment Length Polymorphism (RFLP) Typing of DNA Extracted from Nasal Secretions
Caruso, JF
DNA and Serology Supervisor and Forensic Scientists, Indianapolis-Marion County Forensic Services Agency, IN

Tahir, MA
DNA and Serology Supervisor and Forensic Scientists, Indianapolis-Marion County Forensic Services Agency, IN

Hamby, PP
DNA and Serology Supervisor and Forensic Scientists, Indianapolis-Marion County Forensic Services Agency, IN

Sovinski, SM
DNA and Serology Supervisor and Forensic Scientists, Indianapolis-Marion County Forensic Services Agency, IN

Tahir, UA
Student, Belzer Middle School, IN

(Received 19 May 1994; accepted 23 August 1994)

Abstract

The restriction fragment length polymorphism (RFLP) analysis of blood, semen, and other body fluids, has become increasingly important in violent criminal cases. The identification of additional tissues suitable for comparison with suspected donors has obvious potential benefit. One type of tissue, that found in nasal secretions, has previously received little attention with regards to genetic analysis. We collected blood and nasal secretion samples from eight individuals, subjected them to traditional RFLP typing methods, and analyzed the results using probes for loci D2S44, D1S7, D10S28, D4S139, and D17S79. All nasal samples provided high DNA yields and hybridization results that matched the corresponding blood standards. Thus, nasal secretions are shown to have potentially significant evidentiary value.

Keywords:
RFLP, DNA typing, nasal secretions, mucus

Paper ID: JFS13804J
DOI: 10.1520/JFS13804J
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