Volume 40, Issue 3 (May 1995)

    Postmortem Drug Metabolism by Bacteria

    (Received 2 June 1994; accepted 12 September 1994)

    CODEN: JFSOAD

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    Abstract

    Studies were undertaken to determine the possible role of enteric bacteria in the postmortem bioconversion of the nitrobenzodiazepines flunitrazepam, clonazepam, and nitrazepam. Flunitrazepam, clonazepam, and nitrazepam were completely metabolized in blood in the presence of eight species of enteric bacteria to their respective 7-amino-metabolites. The rates of metabolism, at 37°C, ranged from 0.1 ng/mL/min for Streptococcus faecalis to 8.8 ng/mL/min for Clostridium perfringens. The rate of conversion was reduced to 87% by a combination of 0.7% (w/v) sodium fluoride and potassium oxalate, and almost completely inhibited (96%) by 1% (w/v) sodium fluoride. pH had variable effects on the rate of metabolic bioconversion of nitrobenzodiazepines, while increasing temperatures were found to generally increase the rate of nitrobenzodiazepine bioconversion. These data support the proposal that bacteria may mediate postmortem bioconversion of the nitrobenzodiazepines.


    Author Information:

    Robertson, MD
    Ph.D. Candidate and Director of Scientific Services, Victorian Institute of Forensic Pathology and Department of Forensic Medicine, Monash University, South Melbourne,

    Drummer, OH
    Ph.D. Candidate and Director of Scientific Services, Victorian Institute of Forensic Pathology and Department of Forensic Medicine, Monash University, South Melbourne,


    Stock #: JFS13791J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13791J

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    Author
    Title Postmortem Drug Metabolism by Bacteria
    Symposium , 0000-00-00
    Committee E30