ISSN: 0022-1198
CODEN: JFSCA
Page Count: 5

Evaluation of the Abuscreen Online Assay for Amphetamines on the Hitachi 737: Comparison with EMIT and GC/MS Methods
Escoto, B
Group Leader, Toxicology Section, Damon Reference Laboratories, CA

Baker, DP
Technical Director, Utak Laboratories, Inc., CA

Caldarone, ME
Group Leader, Toxicology Section, Damon Reference Laboratories, CA

Murphy, MS
Senior Laboratory Assistants, Toxicology Section, Damon Reference Laboratories, CA

Shepp, PF
Senior Laboratory Assistants, Toxicology Section, Damon Reference Laboratories, CA

Salamone, SJ
Director of Research and Product Development, International Drug Monitoring Business Unit, Roche Diagnostic Systems, NJ

Royo, VR
Senior Laboratory Assistants, Toxicology Section, Damon Reference Laboratories, CA

(Received 24 February 1994; accepted 25 May 1994)

Abstract

The performance of the ONLINE Assay for Amphetamines on the Hitachi 737 was compared to the Syva Emit d.a.u. Assay and GC/MS. Randomly screened (n = 2964) patient urine samples were assayed using ONLINE and Emit d.a.u. assays concurrently, using d-amphetamine, 1000 ng/mL and d-methamphetamine, 1000 ng/mL as the screening cutoff for ONLINE and Emit d.a.u. assays, respectively. All presumptive positives were confirmed by GC/MS. The specificity was 99% (2937/2964) for ONLINE and 97% (2873/2964) for Emit. Agreement with GC/MS was 80% (110/137) for ONLINE and 55% (110/201) for Emit.

Keywords:
toxicology, amphetamines, methamphetamines, immunoassay, cost, ONLINE, Emit

Paper ID: JFS13771J
DOI: 10.1520/JFS13771J
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