Hair Analysis for Buprenorphine and Its Dealkylated Metabolite by RIA and Confirmation by LC/ECD

    Volume 39, Issue 6 (November 1994)

    ISSN: 0022-1198

    CODEN: JFSOAD

    Page Count: 7


    Jamey, C
    Forensic Toxicologists, and Professor of Legal Medicine, Institut de Médecine Légale, Strasbourg,

    Kintz, P
    Forensic Toxicologists, and Professor of Legal Medicine, Institut de Médecine Légale, Strasbourg,

    Mangin, P
    Forensic Toxicologists, and Professor of Legal Medicine, Institut de Médecine Légale, Strasbourg,

    Cirimele, V
    Forensic Toxicologists, and Professor of Legal Medicine, Institut de Médecine Légale, Strasbourg,

    Edel, Y
    Psychiatrist Centre Hospitalier Spécialisé, Brumath,

    (Received 28 February 1994; accepted 16 May 1994)

    Abstract

    Hair samples were obtained from 14 subjects admitted 2 or 3 months previously to a detoxification center. All reported an history of intravenous heroin abuse. After decontamination by two dichloromethane washes, about 50 mg hair were pulverized in a ball mill and incubated at 56°C overnight in 1 mL 0.1 HCl.

    After neutralization, buprenorphine analyzed by RIA was in the range of 0.01 to 0.47 ng/mg. To confirm buprenorphine, liquid chromatography was used. After neutralization, drugs were extracted with toluene at pH 8.5 during a 3-step extraction procedure. A portion of the reconstituted residue was injected into a Lichrosorb CN column, with a mobile phase of phosphate buffer (pH 4.0)-acetonitrile-1-heptane sulfonic acid-butylamine (85:17:2:0.01, v/v).

    Detection was achieved by coulometry, and the potential of the electrodes was 0.15 and 0.50 V, respectively.

    Linear calibration curves were obtained from 0.02 to 2.0 ng/mg with a correlation coefficient r > 0.99 for both drugs. The detection limit for the major metabolite was about 0.01 ng/mg and 0.02 ng/mg for buprenorphine, using a 50 mg hair sample. Recovery (at 0.2 ng/mg) was 54 and 62% for norbuprenorphine and buprenorphine, respectively. Drugs concentrations in hair were in the range 0.02–0.59 and not detected—0.15 ng/mg for buprenorphine and norbuprenorphine, respectively. Results suggest that a dose-reponse relationship exists between the concentration of buprenorphine in hair and the administered dose.


    Paper ID: JFS13736J

    DOI: 10.1520/JFS13736J

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    Title Hair Analysis for Buprenorphine and Its Dealkylated Metabolite by RIA and Confirmation by LC/ECD
    Symposium , 0000-00-00
    Committee E30