Journal Published Online: 01 November 1994
Volume 39, Issue 6

Anomalous Migration of PCR Products Using Nondenaturing Polyacrylamide Gel Electrophoresis: The Amelogenin Sex-Typing System

CODEN: JFSCAS

Abstract

Sex-typing of biological samples can be accomplished using the polymerase chain reaction (PCR) to amplify DNA sequences that are specific for the Y-chromosome. One such system is based on PCR amplification of the X-chromosome amelogenin gene and the amelogenin-like sequences located near the centromere of the Y-chromosome. The X and Y PCR products can be distinguished from each other on the basis of a 177 basepair (bp) insertion in the X relative to the Y. In this report, we demonstrate that the amelogenin PCR products migrate anomalously using non-denaturing polyacrylamide gel electrophoresis (ND-PAGE) as opposed to agarose gel electrophoresis or denaturing PAGE. These results may be relevant to the choice of electrophoretic system used to analyze highly polymorphic loci for individual identification.

Author Information

Eng, B
DNA Diagnostic Laboratory, McMaster University Medical Centre, Hamilton, Ontario, Canada
Ainsworth, P
DNA Diagnostic Laboratory, Victoria Hospital/Children's Hospital of Western Ontario, London, Ontario, Canada
Waye, JS
McMaster University, Hamilton, Ontario, Canada
Pages: 4
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Stock #: JFS13724J
ISSN: 0022-1198
DOI: 10.1520/JFS13724J