(Received 22 February 1994; accepted 17 May 1994)
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Sex-typing of biological samples can be accomplished using the polymerase chain reaction (PCR) to amplify DNA sequences that are specific for the Y-chromosome. One such system is based on PCR amplification of the X-chromosome amelogenin gene and the amelogenin-like sequences located near the centromere of the Y-chromosome. The X and Y PCR products can be distinguished from each other on the basis of a 177 basepair (bp) insertion in the X relative to the Y. In this report, we demonstrate that the amelogenin PCR products migrate anomalously using non-denaturing polyacrylamide gel electrophoresis (ND-PAGE) as opposed to agarose gel electrophoresis or denaturing PAGE. These results may be relevant to the choice of electrophoretic system used to analyze highly polymorphic loci for individual identification.
Technologist, DNA Diagnostic Laboratory, McMaster University Medical Centre, Hamilton, Ontario,
Co-Director, DNA Diagnostic Laboratory, McMaster University Medical Centre, Hamilton, Ontario
Director, DNA Diagnostic Laboratory, Victoria Hospital/Children's Hospital of Western Ontario, London, Ontario
Stock #: JFS13724J