Volume 39, Issue 6 (November 1994)

    Anomalous Migration of PCR Products Using Nondenaturing Polyacrylamide Gel Electrophoresis: The Amelogenin Sex-Typing System

    (Received 22 February 1994; accepted 17 May 1994)

    CODEN: JFSOAD

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    Abstract

    Sex-typing of biological samples can be accomplished using the polymerase chain reaction (PCR) to amplify DNA sequences that are specific for the Y-chromosome. One such system is based on PCR amplification of the X-chromosome amelogenin gene and the amelogenin-like sequences located near the centromere of the Y-chromosome. The X and Y PCR products can be distinguished from each other on the basis of a 177 basepair (bp) insertion in the X relative to the Y. In this report, we demonstrate that the amelogenin PCR products migrate anomalously using non-denaturing polyacrylamide gel electrophoresis (ND-PAGE) as opposed to agarose gel electrophoresis or denaturing PAGE. These results may be relevant to the choice of electrophoretic system used to analyze highly polymorphic loci for individual identification.


    Author Information:

    Eng, B
    Technologist, DNA Diagnostic Laboratory, McMaster University Medical Centre, Hamilton, Ontario,

    Waye, JS
    Co-Director, DNA Diagnostic Laboratory, McMaster University Medical Centre, Hamilton, Ontario

    Ainsworth, P
    Director, DNA Diagnostic Laboratory, Victoria Hospital/Children's Hospital of Western Ontario, London, Ontario


    Stock #: JFS13724J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13724J

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    Title Anomalous Migration of PCR Products Using Nondenaturing Polyacrylamide Gel Electrophoresis: The Amelogenin Sex-Typing System
    Symposium , 0000-00-00
    Committee E30