Volume 39, Issue 5 (September 1994)

    Amplification of cDNA via RT-PCR Using RNA Extracted from Postmortem Tissues

    (Received 30 August 1993; accepted 7 March 1994)

    CODEN: JFSOAD

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    Abstract

    Analysis of cDNA derived from messenger RNA is of advantage over using genomic DNA in genetic analysis of large genes, especially those with lengthy intron sequences. However, because of its instability and rapid degradation, RNA extraction from postmortem tissues has not been attempted. Here, we report the successful extraction of intact mRNA from various postmortem tissues from accidental and sudden death cases. Subsequently with reverse transcriptase-polymerase chain reaction (RT-PCR), we were able to amplify cDNA fragments of different lengths up to 0.9 kb. The described method therefore provides a useful tool in genetic analysis of postmortem tissues.


    Author Information:

    Ong, C-N
    Research Assistant, Research Scientist, and Associate Professor, Department of Community, Occupational and Family Medicine,

    Shi, C-Y
    Research Assistant, Research Scientist, and Associate Professor, Department of Community, Occupational and Family Medicine,

    Chia, J-N
    Laboratory Technician,

    Phang, T-W
    Research Assistant, Research Scientist, and Associate Professor, Department of Community, Occupational and Family Medicine,


    Stock #: JFS13713J

    ISSN: 0022-1198

    DOI: 10.1520/JFS13713J

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    Author
    Title Amplification of cDNA via RT-PCR Using RNA Extracted from Postmortem Tissues
    Symposium , 0000-00-00
    Committee E30