Volume 39, Issue 3 (May 1994)
Anatomy and Viability of Cannabis sativa Stem Cuttings With and Without Adventitious Roots
The objective of this research was to determine the stage of root development of Cannabis sativa stem cuttings necessary to produce viable plants. Cuttings were made from one pistillate (female) plant and transplanted into pots when callus, newly emerging roots, or 1 to 2 mm long roots were present. Cuttings were grown for two weeks and survival and biomass accumulation were recorded. Cross sections of the stems and roots of cuttings and roots of seven-day-old seedlings were made for anatomical comparisons. Based on the cross sections, the general anatomical features of roots from seedlings and cuttings were similar. As in some other herbaceous species, adventitious roots of C. sativa stem cuttings develop from the vascular cambium. Two weeks after transplanting, all propagules from the three cutting types survived and grew. Total biomass accumulation and components of total biomass accumulation of the cuttings were not significantly different among cutting types. Thus, cuttings of C. sativa at the callus stage are capable of survival and growth comparable to rooted cuttings without any additional care beyond that normally given to plants established from seed.