ISSN: 0022-1198
CODEN: JFSCA
Page Count: 8

Potential Forensic Applications of Minisatellite Variant Repeat (MVR) Mapping Using the Polymerase Chain Reaction (PCR) at D1S8
Tamaki, K
Assistant Professors, Research Student, Assistant Professor and Professor, Nagoya University School of Medicine,

Uchihi, R
Assistant Professors, Research Student, Assistant Professor and Professor, Nagoya University School of Medicine,

Kojima, T
Assistant Professors, Research Student, Assistant Professor and Professor, Nagoya University School of Medicine,

Yamamoto, T
Assistant Professors, Research Student, Assistant Professor and Professor, Nagoya University School of Medicine,

Katsumata, Y
Assistant Professors, Research Student, Assistant Professor and Professor, Nagoya University School of Medicine,

(Received 23 August 1993; accepted 12 November 1993)

Abstract

Minisatellite variant repeat (MVR) mapping using the polymerase chain reaction (PCR) at D1S8 (MS32) was applied to samples from various human tissues. All DNA samples obtained from an individual's organs at autopsy consistently gave the same digital diploid codes. Even 1 ng of genomic DNA was sufficient to obtain authentic diploid MVR coding ladders. MVR-PCR could be reliably applied to DNA isolated from bloodstains, saliva stains, seminal stains and plucked hair roots, and should become a powerful tool for individual identification in forensic investigations.

Keywords:
pathology and biology, individual identification, minisatellite, polymerase chain reaction, digital DNA typing

Paper ID: JFS13651J
DOI: 10.1520/JFS13651J
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